FastQCFastQC Report
Thu 14 Sept 2023
SRR14323352_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14323352_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65719049
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length43-61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1133511717.247840880959796Illumina PCR Primer Index 6 (96% over 29bp)
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2741670.4171804129423723Illumina PCR Primer Index 6 (96% over 29bp)
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA2127190.3236793642586033Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1240240.18871849469398136Illumina PCR Primer Index 6 (100% over 28bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA971810.14787341186267014Illumina PCR Primer Index 6 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)