FastQCFastQC Report
Thu 14 Sept 2023
SRR14323358_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14323358_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20731056
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length2-63
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA414650.20001393079059743Illumina PCR Primer Index 6 (96% over 29bp)
CTTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA318730.15374518307219853Illumina PCR Primer Index 6 (96% over 29bp)
ATTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA304720.14698720605452997Illumina PCR Primer Index 6 (96% over 29bp)
TATTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA278430.13430574882437246Illumina PCR Primer Index 6 (96% over 29bp)
GTTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA239030.11530044586247801Illumina PCR Primer Index 6 (96% over 29bp)
TTCTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA233360.11256541876110894Illumina PCR Primer Index 6 (96% over 29bp)
TTTCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA228640.11028864135044544Illumina PCR Primer Index 6 (96% over 29bp)
TCTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA222500.10732690124420097Illumina PCR Primer Index 6 (96% over 29bp)
TTATCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA217250.10479446874293331Illumina PCR Primer Index 6 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)