FastQCFastQC Report
Thu 7 Sept 2023
SRR14323359_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14323359_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23856586
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length1-63
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA363880.15252811110525202Illumina PCR Primer Index 6 (96% over 29bp)
CTTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA286160.11995010518269464Illumina PCR Primer Index 6 (96% over 29bp)
ATTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA277250.1162152874682069Illumina PCR Primer Index 6 (96% over 29bp)
TATTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA274210.11494100622779806Illumina PCR Primer Index 6 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)