FastQCFastQC Report
Thu 14 Sept 2023
SRR14323362_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14323362_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47285635
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length28-62
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA25397705.371123809588261Illumina PCR Primer Index 6 (96% over 29bp)
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1101400.23292486185286504Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTTAAAAAAAAA699970.14803015757322494Illumina PCR Primer Index 6 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)