FastQCFastQC Report
Thu 14 Sept 2023
SRR14323368_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14323368_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97704720
Total Bases7.4 Gbp
Sequences flagged as poor quality0
Sequence length2-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1828840.18718031227150542Illumina PCR Primer Index 6 (96% over 29bp)
CTTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1233000.12619656450578845Illumina PCR Primer Index 6 (96% over 29bp)
ATTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1171280.11987957183644761Illumina PCR Primer Index 6 (96% over 29bp)
TATTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1117880.11441412451721882Illumina PCR Primer Index 6 (96% over 29bp)
TTCTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1045680.10702451222417914Illumina PCR Primer Index 6 (96% over 29bp)
TTTCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1032150.10563972753823972Illumina PCR Primer Index 6 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)