FastQCFastQC Report
Thu 7 Sept 2023
SRR14323388_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14323388_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32935328
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length39-61
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA672745120.42624564115469Illumina PCR Primer Index 6 (96% over 29bp)
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA4686991.4230889092709202Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA2634160.7997977126567557Illumina PCR Primer Index 6 (100% over 28bp)
CGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1823910.5537852849074404Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1202060.36497587028737044Illumina PCR Primer Index 6 (100% over 28bp)
CAGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA470170.14275552379499606Illumina PCR Primer Index 6 (96% over 29bp)
ATGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA439010.1332945583538746Illumina PCR Primer Index 6 (100% over 28bp)
CTTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA424590.12891628102200775Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCATCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA342410.10396435098505774Illumina PCR Primer Index 6 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)