FastQCFastQC Report
Thu 14 Sept 2023
SRR14323398_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14323398_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88478556
Total Bases6.7 Gbp
Sequences flagged as poor quality0
Sequence length1-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA3133800.35418751635141965Illumina PCR Primer Index 6 (96% over 29bp)
CTTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA2212650.2500775442130859Illumina PCR Primer Index 6 (96% over 29bp)
ATTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA2052540.2319816340583135Illumina PCR Primer Index 6 (96% over 29bp)
TATTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA2031610.22961608912333517Illumina PCR Primer Index 6 (96% over 29bp)
TTCTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1757190.19860066432368087Illumina PCR Primer Index 6 (96% over 29bp)
TTTCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1693260.19137518473967863Illumina PCR Primer Index 6 (96% over 29bp)
TTATCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1670300.1887802056805719Illumina PCR Primer Index 6 (96% over 29bp)
TCTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1643720.185776087937059Illumina PCR Primer Index 6 (96% over 29bp)
GTTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1475150.16672401389552516Illumina PCR Primer Index 6 (96% over 29bp)
TGTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1439720.16271965378820152Illumina PCR Primer Index 6 (96% over 29bp)
CCTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1336970.1511066704117549Illumina PCR Primer Index 6 (96% over 29bp)
TTTGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1315340.14866201026155987Illumina PCR Primer Index 6 (96% over 29bp)
CATTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1302870.14725262921334295Illumina PCR Primer Index 6 (96% over 29bp)
TACTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1282020.1448961260172465Illumina PCR Primer Index 6 (96% over 29bp)
CTATCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1189660.13445743847808728Illumina PCR Primer Index 6 (96% over 29bp)
AATTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1186620.13411385240057488Illumina PCR Primer Index 6 (96% over 29bp)
TTGTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1184830.13391154349309228TruSeq Adapter, Index 6 (96% over 29bp)
CTCTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1175170.13281975352310227Illumina PCR Primer Index 6 (96% over 29bp)
ATATCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1157390.1308102270565989Illumina PCR Primer Index 6 (96% over 29bp)
ACTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1140080.12885382080602673Illumina PCR Primer Index 6 (96% over 29bp)
TCCTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1136070.12840060364457123Illumina PCR Primer Index 6 (96% over 29bp)
TTACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1116760.12621815392195143Illumina PCR Primer Index 6 (96% over 29bp)
ATCTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1077070.12173232121916637Illumina PCR Primer Index 6 (96% over 29bp)
TAATCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1067070.12060210385892826Illumina PCR Primer Index 6 (96% over 29bp)
ATTCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1053370.11905370607540205Illumina PCR Primer Index 6 (96% over 29bp)
TATCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1051700.11886495977624228Illumina PCR Primer Index 6 (96% over 29bp)
TTCCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA996100.11258095125331838Illumina PCR Primer Index 6 (96% over 29bp)
TGCTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA977570.11048665848479716Illumina PCR Primer Index 6 (96% over 29bp)
GCTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA968000.10940504047104928Illumina PCR Primer Index 6 (96% over 29bp)
TCATCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA934790.10565158861769852Illumina PCR Primer Index 6 (96% over 29bp)
CTTCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA932140.10535208101723542Illumina PCR Primer Index 6 (96% over 29bp)
GATTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA929960.10510569363270351Illumina PCR Primer Index 6 (96% over 29bp)
ATACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA926250.10468638299205517Illumina PCR Primer Index 6 (96% over 29bp)
CTTGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA900860.1018167611144106Illumina PCR Primer Index 6 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)