FastQCFastQC Report
Thu 14 Sept 2023
SRR14323399_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14323399_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86151490
Total Bases6.5 Gbp
Sequences flagged as poor quality0
Sequence length3-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1345990.1562352548980871Illumina PCR Primer Index 6 (96% over 29bp)
CTTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA905560.10511251749679545Illumina PCR Primer Index 6 (96% over 29bp)
ATTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA870430.10103481669324582Illumina PCR Primer Index 6 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)