FastQCFastQC Report
Thu 14 Sept 2023
SRR14323400_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14323400_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19745464
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length2-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA552340.2797300686375362Illumina PCR Primer Index 6 (96% over 29bp)
CTTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA389890.19745800858364232Illumina PCR Primer Index 6 (96% over 29bp)
ATTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA353660.17910949066580556Illumina PCR Primer Index 6 (96% over 29bp)
TATTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA341610.1730068232379852Illumina PCR Primer Index 6 (96% over 29bp)
TTCTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA307950.15595987007446369Illumina PCR Primer Index 6 (96% over 29bp)
TTTCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA307710.15583832317133697Illumina PCR Primer Index 6 (96% over 29bp)
TTATCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA295530.14966981783765626Illumina PCR Primer Index 6 (96% over 29bp)
TCTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA278380.14098427871839325Illumina PCR Primer Index 6 (96% over 29bp)
GTTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA257100.13020711997449136Illumina PCR Primer Index 6 (96% over 29bp)
TTTGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA254080.12867765477681353Illumina PCR Primer Index 6 (96% over 29bp)
TGTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA249300.12625684562287318Illumina PCR Primer Index 6 (96% over 29bp)
CCTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA242310.12271679206930766Illumina PCR Primer Index 6 (96% over 29bp)
CATTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA233200.11810307420478952Illumina PCR Primer Index 6 (96% over 29bp)
TACTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA219360.11109386945781573Illumina PCR Primer Index 6 (96% over 29bp)
CTATCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA216190.10948843744568372Illumina PCR Primer Index 6 (96% over 29bp)
TTGTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA212200.10746772018120213TruSeq Adapter, Index 6 (96% over 29bp)
CTCTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA209270.10598383507219684Illumina PCR Primer Index 6 (96% over 29bp)
AATTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA204170.10340096338075419Illumina PCR Primer Index 6 (96% over 29bp)
ACTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA199680.10112702340142525Illumina PCR Primer Index 6 (96% over 29bp)
ATATCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA199560.1010662499498619Illumina PCR Primer Index 6 (96% over 29bp)
TCCTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA198110.10033190407680469Illumina PCR Primer Index 6 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)