FastQCFastQC Report
Thu 14 Sept 2023
SRR14323401_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14323401_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53913386
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length2-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1585030.29399563217936264Illumina PCR Primer Index 6 (96% over 29bp)
ATTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1132940.2101407617024833Illumina PCR Primer Index 6 (96% over 29bp)
CTTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1095410.20317959625091994Illumina PCR Primer Index 6 (96% over 29bp)
TATTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA1047020.19420408875821674Illumina PCR Primer Index 6 (96% over 29bp)
TTTCCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA833060.15451821185929596Illumina PCR Primer Index 6 (96% over 29bp)
TTCTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA794030.1472788223689011Illumina PCR Primer Index 6 (96% over 29bp)
TTATCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA771480.14309618765180135Illumina PCR Primer Index 6 (96% over 29bp)
TCTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA756510.1403195117442633Illumina PCR Primer Index 6 (96% over 29bp)
GTTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA749180.13895992360784015Illumina PCR Primer Index 6 (96% over 29bp)
TGTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA671870.12462025664646624Illumina PCR Primer Index 6 (96% over 29bp)
CATTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA661570.12270978491315682Illumina PCR Primer Index 6 (96% over 29bp)
TACTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA647620.12012230135202415Illumina PCR Primer Index 6 (96% over 29bp)
AATTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA631020.11704328865562257Illumina PCR Primer Index 6 (96% over 29bp)
CCTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA618720.11476185153720452Illumina PCR Primer Index 6 (96% over 29bp)
TTTGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA606950.11257872024584026Illumina PCR Primer Index 6 (96% over 29bp)
ATATCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA600260.1113378410326519Illumina PCR Primer Index 6 (96% over 29bp)
CTATCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA569700.1056694899481921Illumina PCR Primer Index 6 (96% over 29bp)
ACTTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA562420.1043191759463967Illumina PCR Primer Index 6 (96% over 29bp)
TTGTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA549590.10193943300092485TruSeq Adapter, Index 6 (96% over 29bp)
CTCTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA549040.10183741752001998Illumina PCR Primer Index 6 (96% over 29bp)
TTACCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA541620.10046113594126699Illumina PCR Primer Index 6 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)