FastQCFastQC Report
Thu 14 Sept 2023
SRR14340945_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14340945_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10635934
Total Bases309.9 Mbp
Sequences flagged as poor quality0
Sequence length15-147
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTGGGCCGGCGGGGCTTGGGCGAAAGCTCT2168982.039294339359383No Hit
AGTAGTGGGCCGGCGGGGCTTGGGCGAAAGCTCT1550961.4582264237442617No Hit
GCCGCCATGGTGAAATTGGTAGACACGCTGCTC1335271.2554327621814878No Hit
GCGCTCTTAGTTCAGTTCGGTAGAACGCGGG1223801.1506276740716894No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCC1214141.141545255922047No Hit
GCCGCCATGGTGAAATTGGTAGACACGCTG1188961.1178707953622127No Hit
TCCCAGTAAAACGCGGCGTGTTCGAATG825540.7761800703163446No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACT751470.7065387957465701No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACTC714230.6715254156334555No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCT589570.5543189718928304No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCC520720.4895855878759684No Hit
TCATGCATTGGATGGATGCCCGGGCATTGAGA481770.4529644505127617No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACCACTGTATCTCGTATGCCG477050.44852666441894057TruSeq Adapter, Index 1 (97% over 34bp)
GCCCCTATCGTCTAGTGGTTCAGGACA440850.41449110158073565No Hit
AGTGGGCCGGCGGGGCTTGGGCGAAAGCTCT436970.4108430909781877No Hit
GGGTGTTTGGTCTAGTGGTATGATTCTCGCT435660.40961141729536865No Hit
AGGGATGTAGCGCAGCTTGGTAGCGCTTTTGTT345850.32517125435340233No Hit
TTGTAGTCTAGCTGGTTAGGATACTCGG327640.3080500499532998No Hit
GTAGTGGGCCGGCGGGGCTTGGGCGAAAGCTCC323260.30393193489166065No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCCT300600.2826268008056462No Hit
GGGCCTGTAGCTCAGAGGATTAGAGCACGTGG294910.27727701206118804No Hit
TGGACGTCATAATTTGTGGTAGAGGG291340.27392046622327665No Hit
GCCGCCATGGTGAAATTGGTAGACACGCTGCTT282720.2658158653485439No Hit
TTGTCGTCCAGCGGTTAGGATATCTGG270430.2542606977440815No Hit
AGCACATGACGAAGTGAGCGGGCTCT238450.22419281654060658No Hit
AGTAGTGGGCCGGCGGGGCTTGGGCGAAAGCTCC223230.2098828368058696No Hit
TCCCAGTAAAACGCGGCGTGTTCGAAT196930.18515534225767102No Hit
GCCGCCATGGTGAAATTGGTAGACACGCTGCTCT195620.183923668574852No Hit
GCCCCTATCGTCTAGTGGTTCAGGACATCTCT193270.18171417761712322No Hit
GGGATTGTAGTTCAATTGGTCAGAGCACCGCA184310.17328990571020844No Hit
TCCGTTGTCGTCCAGCGGTTAGGATATCTGG172450.1621390279405645No Hit
TTGACTACTGAACCATGCCTGGATCGT168410.1583405839111074No Hit
GTCAGGATAGCTCAGTTGGTAGAGCAGAGG166350.15640375353965152No Hit
GCGAGCGTAGTTCAATGGTAAAACATCTCCTTG160870.15125140866801165No Hit
GGGCCTGTAGCTCAGAGGATTAGAGCACGT159340.1498128890231925No Hit
TCCGTTGTAGTCTAGCTGGTTAGGAT157470.1480546983461913No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATA151760.14268610542336949No Hit
GCCGCCATGGTGAAATTGGTAGACACGCTGCT151020.14199035082391448No Hit
GTAGTGGGCCGGCGGGGCTTGGGCGAAAGCTCA149800.1408432959437319No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACTT145630.13692262475491104No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACC142590.13406438964363637No Hit
GCCGCCATGGTGAAATTGGTAGACACGCTA140990.13256005537454443No Hit
TAGTGGGCCGGCGGGGCTTGGGCGAAAGCTCT135340.12724787498681359No Hit
TTGTCGTCTAGTCCGGTTAGGATACCTGG129390.12165363192362796No Hit
GGGCCTGTAGCTCAGAGGATTAGAGCACGTG122750.11541064470689645No Hit
GGGAGAATCTTAGCGACAAAGGGCTA121980.11468668383989597No Hit
GGGAGAGGGATGCAACACGAGGAC118800.11169681948007575No Hit
GGCGCGTCGAGAGAACCATGTCGAAGGAAC118430.11134894218034824No Hit
AGGGCTATAGCTCAGTTCGGTAGAGCAACTCGT117430.11040873326216577No Hit
GGGGATATGGCGAAATCGGTAGACGCT115290.10839668617725533No Hit
AGCACATGACGAAGTGAGCGGGCTCTT114800.10793598380734594No Hit
GCGCTCTTAGTTCAGTTCGGTAGAACGCGGA113960.10714620831607266No Hit
AGAACGTGAAACCGTGCTGAGCTCCCAAGC112680.10594274090079912No Hit
GGCGCGTCGAGAGAACCATGTCGAAGGAACT111200.1045512317018891No Hit
AGTAGTGGGCCGGCGGGGCTTGGGCGAAAGCTCA107880.10142973809352333No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)