FastQCFastQC Report
Thu 14 Sept 2023
SRR14340950_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14340950_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6391933
Total Bases179.5 Mbp
Sequences flagged as poor quality0
Sequence length16-42
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATACGACCATAGGTAGAGGAGAACT4315476.7514318438569365No Hit
GTAGTGGGCCGGCGGGGCTTGGGCGAAAGCTCT1227111.919779196684321No Hit
ACATACGACCATACCCACTGGAGAACTCGGGAT1024741.6031770045149096No Hit
ACATACGACCATAGGTAGAGGAGAACC467380.7312029084159675No Hit
ACATACGACCATAGGTAGAGGAGAAC374620.5860824886618805No Hit
TAGTGGGCCGGCGGGGCTTGGGCGAAAGCTCT374090.5852533185188268No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACTC315310.4932936562382616No Hit
ACATACGACCATAGGTAGAGGAGAACTCGGGAT304970.4771170160888733No Hit
GTAGTGGGCCGGCGGGGCTTGGGCGAAAGCTC303600.4749736894926777No Hit
TCCGTTGTAGTCTAGCTGGTTAGGAT299260.4681838811514451No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATA254800.3986274574530115No Hit
AGTGGGCCGGCGGGGCTTGGGCGAAAGCTCT249560.3904296243405555No Hit
ACATACGACCATAGGGTGTGGAAAACAGG234620.36705641313824783No Hit
ACATACGACCATAGGTAGAGGAGAACTCGGGATC233270.3649443759814128No Hit
ACATACGACCATACCCATTGGAAAACTCGGGAT219480.343370307542335No Hit
GGGTGTTTGGTCTAGTGGTATGATTCTCGCT218960.3425567821189615No Hit
ACATACGACCATACCCACTGGAGAACT207490.3246122886457039No Hit
ACATACGACCATACCTATCAGAGAACTCGGGAT204720.320278701294272No Hit
ACATACGACCATACCCACTGGAGAACTCGGGA201570.31535061459499025No Hit
ACATACGACCATAGGTAGAGGAGA178950.2799622586782433No Hit
TTTTACCGTACTGCAAACCGCCACAGGTGGACA175730.2749246589411998No Hit
TTTTACCGTACTGCAAACCGCCACAGGTGGAC167520.26208034408370673No Hit
ACATACGACCATAGGTAGAGGAGAACTCGGG153460.24008386821326191No Hit
TGAGTGTAAACCAACGACGTCAGGCCGA148550.23240231085025453No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATACT145080.22697359312120452No Hit
TTTACCGTACTGCAAACCGCCACAGGTGGACA141370.22116940211982825No Hit
ACATACGACCATAGGTAGAGGAGAACTCGGGA137710.21544343471685323No Hit
GTAGTGGGCCGGCGGGGCTTGGGCGAAAGCTCC135710.21231449078080136No Hit
ACATACGACCATAGGGTGTGGAAAACAGGGCT135710.21231449078080136No Hit
TCCGGTATGGTGTAGTGGCTAACATTTCGCGCT126730.19826553250792836No Hit
ACATACGACCATAGGTAGAGGAGAACTCG125210.19588753511652893No Hit
TCCGGTATGGTGTAGTGGCTAACATT123890.19382243211873465No Hit
GCGTCTGTAGTCCAACGGTTAGGATAATTGTC114410.17899123786184867No Hit
TTTACCGTACTGCAAACCGCCACAGGTGGAC113640.17778659444646872No Hit
ACATACGACCATAGGTAGAGGAGAACTCGG111650.17467329523009706No Hit
ACATACGACCATACCCACTGGAGAACTCGGG107790.16863443343351692No Hit
ACATACGACCATACCCATTGGAGAACTCGGGAT103680.16220445364493025No Hit
ACATACGACCATAGGTAGAGGAGAA102460.1602957978439386No Hit
ACATACGACCATACCCACTGGAGAAC102300.16004548232905447No Hit
TCCGGTATGGTGTAGTGGCTAACATTTC99900.15629074960579217No Hit
TAGTGGGCCGGCGGGGCTTGGGCGAAAGCTC99240.15525819810689503No Hit
GGGAGACCATCGACTACGCCTTTCGGCCTGA96800.15144088650491175No Hit
ACATACGACCATACCCACTGGAGAACTCGGGAC95340.14915675743159387No Hit
ACATACGACCATACCCACTGGAAAACTCGGGAT89550.1400984647367236No Hit
ACATACGACCATAGGGTGTGGAAAACAGGGCTT83970.13136871115513885No Hit
TCCGTTGTAGTCTAGCTGGTTAGGATAC81860.12806767530260407No Hit
TTCCCGGGAGCATGCCCGTCTGAGCGTCGTT81030.12676916356914253No Hit
GCATCATTGGTCTAGTGGTAGAATTCGTCGTT76560.11977597387206657No Hit
ACATACGACCATAGGTAGAGGAGAACA76380.11949436891782188No Hit
ACATACGACCATACCTATCAGAAAACTCGGGAT70420.11017011598838723No Hit
ACATACGACCATACCTATCAGAGAACT69940.1094191694437348No Hit
CACCGTGTAATACCCTGTGCTGGACTGAGGT68130.10658747518160783No Hit
GGTGGCTGTAGTTTAGTGGTGAGAATTCC68000.10638409382576444No Hit
TCTCTGATAGTATAATGGTCAGTATACC67680.10588346279599614No Hit
GGGAGACCATCGACTACGCCTTTCGGCCTGATC66510.10405303059340579No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)