FastQCFastQC Report
Thu 14 Sept 2023
SRR14510084_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14510084_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40011718
Total Bases996.4 Mbp
Sequences flagged as poor quality0
Sequence length10-89
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGACTCTAGATAACCTC13222513.3046594000287617No Hit
TTGGTGACTCTAGATAACCTCG12412973.1023336713509777No Hit
AACTGTGGTAATTCTAGAGCT11965632.9905314238193923No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC8607682.1512897796590487No Hit
TTGGTGACTCTAGATAACCT7487321.871281807994348No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA6914301.728068762256097No Hit
ACTGTGGTAATTCTAGAGCT5820821.4547788225439358No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT5320211.329662975231406No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC4869191.2169409971348892No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA4430831.1073830921231624No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG4351761.0876213813163433No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC4223651.0556032610246828No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA3535340.8835761563649928No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT3162590.7904159476481364No Hit
TAACTGTGGTAATTCTAGAGCT2676050.6688165701857641No Hit
TTTGGTGACTCTAGATAACCTCG2382060.5953405949727028No Hit
GAGGGCGCACCACCGGCCCGTCTCGC2371650.5927388571517974No Hit
TTTGGTGACTCTAGATAACCTC2296930.5740643278551548No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATT2136380.5339385826922003No Hit
GAGGGCGCACCACCGGCCCGTCTCG2085950.5213347749776702No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT2075560.5187380356924439No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC1948650.4870198275415217No Hit
TTGGTGACTCTAGATAACCTCGG1630700.4075556065850509No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC1343000.33565167084302655No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG1318580.3295484587790007No Hit
GGTTGGCCTCGGATAGCCGGTC1301130.32518723639909686No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGC1279920.3198862893115462No Hit
CTGTGGTAATTCTAGAGCT1235800.3088595196037321No Hit
CAGTGGCTGGTTGAGAT1220180.3049556632384543No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1174150.2934515333732983No Hit
AGGAATAATGGAAT1151620.2878206829309354No Hit
TTTGGTGACTCTAGATAACCT1057410.26427508061513383No Hit
CCTGCGGAAGGATCATTA1029990.25742208819926204No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGC984500.2460529187974383No Hit
AACTGTGGTAATTCTAGAGCTA974550.24356614729714932No Hit
TAGGAATAATGGAAT944890.23615331888523255No Hit
AGCTAGGAATAATGGAAT889150.22222239994793525No Hit
AGGGAGTCGGGTTCAGATCCCCGAATCCGGAG862660.21560183944113573No Hit
AACTGTGGTAATTCTAGAGC843550.21082573859987716No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCA827670.20685690127077275No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAT809240.20225075064259926No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG804910.20116856766810165No Hit
TTCTAGAGCT798450.19955404064379342No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA768540.1920787305358895No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAA750450.18755755501425858No Hit
TGGTGACTCTAGATAACCTC743760.18588554482964217No Hit
AAAGATTAAGCCATGCATGTCTA739200.18474587869483633No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAC732450.183058872903183No Hit
AACGATGCCGACCGGCGATGCGGCGGCG691040.17270940477986974No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTCG676720.16913045323372516No Hit
CCGGGGTCCGGTGCGGAGTGCCCT668170.16699357923096428No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGG662380.16554650315190164No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTC659920.16493168326338797No Hit
TGGTGACTCTAGATAACCTCG658460.16456679015882297No Hit
TTGGTGACTCTAGATAACC653540.16333715038179566No Hit
TTTGGTGACTCTAGATAACCTCGG638140.15948827790898656No Hit
CCTGCGGAAGGATCATT634820.15865852098627709No Hit
TTGGTGACTCTAGATAACCTCGGGCCG625260.15626922093172807No Hit
TTTTGGTGACTCTAGATAACCTCG620560.15509456504716942No Hit
AAGGGAGTCGGGTTCAGATCCCCGAATCCGGAG614560.1535950043434776No Hit
AGCAGTGGCTGGTTGAGATTT602850.15066836170343897No Hit
AATTAGAGTGTTCAAAGCAGGCCC575360.1437978744126908No Hit
TCGGGCCGATCGCAC574970.14370040296695083No Hit
ATAACCTCGGGCCGATCGCAC572520.14308808234627665No Hit
TCGGGCCGATCGCA565130.1412411234128962No Hit
TTTTGGTGACTCTAGATAACCTC558030.13946664324686084No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC557030.1392167164629122No Hit
AGAGCAGTGGCTGGTTGAGATTTAA553720.13838945880804218No Hit
CGGTTGGCCTCGGATAGCCGGTC548680.13712982781694102No Hit
ACTGTGGTAATTCTAGAGC546680.13662997424904375No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCT545610.13636255259021868No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATA531210.13276360690135824No Hit
ACGATGCCGACCGGCGATGCGGCGGCG511130.12774507707966953No Hit
TAGATAACCTCGGGCCGATCGCAC497120.12424360283654903No Hit
ATAACCTCGGGCCGATCGCA490870.12268156043687004No Hit
CGAGGGCGCACCACCGGCCCGTCTCG489530.12234665854637884No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG473530.11834783000320057No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG466060.11648087692710421No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAA461370.11530872031038507No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC455020.1137216852323112No Hit
CAGCTAGGAATAATGGAAT450130.11249954325880233No Hit
AGATAACCTCGGGCCGATCGCAC449410.11231959597435932No Hit
TGGTGACTCTAGATAACCT448070.11198469408386813No Hit
TTTGGTGACTCTAGATAACCTCGGGCCG442190.11051512459425011No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGC440230.11002526809771078No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCG435180.10876313783877013No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG424130.10600144687613762No Hit
AGGGCGCACCACCGGCCCGTCTCGC411980.10296483645116164No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGA405190.10126783358815035No Hit
TAACTGTGGTAATTCTAGAGCTA401830.10042807959408291No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)