FastQCFastQC Report
Thu 14 Sept 2023
SRR14510085_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14510085_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21493329
Total Bases540.4 Mbp
Sequences flagged as poor quality0
Sequence length10-89
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGACTCTAGATAACCTCG8460603.9363841683156666No Hit
AACTGTGGTAATTCTAGAGCT6780723.154802124882562No Hit
TTGGTGACTCTAGATAACCTC5663072.6348035709126307No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC3783611.7603648090065527No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA3475241.616892385539718No Hit
ACTGTGGTAATTCTAGAGCT3282351.5271482607463926No Hit
TTGGTGACTCTAGATAACCT3055141.4214363907982797No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT3036411.4127220590165441No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG2852251.3270396596078717No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA2608301.213539326551043No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2534511.1792077439469706No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC2112800.9830026795756022No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA1967640.915465445115552No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT1873780.871796081472535No Hit
TTTGGTGACTCTAGATAACCTCG1724420.80230475232571No Hit
TAACTGTGGTAATTCTAGAGCT1531510.7125513223195904No Hit
GAGGGCGCACCACCGGCCCGTCTCG1485370.6910841964034515No Hit
GAGGGCGCACCACCGGCCCGTCTCGC1419310.6603490785443242No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATT1324550.6162609803255699No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT1255260.5840230706001848No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC1126440.5240881949929673No Hit
TTTGGTGACTCTAGATAACCTC1032700.48047466262671545No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC890760.4144355674265257No Hit
TTGGTGACTCTAGATAACCTCGG847210.3941734665672311No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGC826590.38457979217644694No Hit
CTGTGGTAATTCTAGAGCT759380.35330962458165505No Hit
GGTTGGCCTCGGATAGCCGGTC741120.34481396530058234No Hit
CCTGCGGAAGGATCATTA685570.31896873676478876No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGC647060.3010515495296238No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG635380.2956173052578314No Hit
AGGGAGTCGGGTTCAGATCCCCGAATCCGGAG564400.2625931050513394No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC564350.262569842019354No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAT516690.24039551993085853No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCA507530.23613373247113092No Hit
AACTGTGGTAATTCTAGAGCTA496300.23090885548720722No Hit
TGGTGACTCTAGATAACCTCG495130.23036450053874857No Hit
AGGAATAATGGAAT469300.2183468182150843No Hit
TTTTGGTGACTCTAGATAACCTCG466820.21719297182860786No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAA465960.21679284767845874No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTCG455450.211902958355125No Hit
TTTGGTGACTCTAGATAACCT453900.21118180436357717No Hit
AATTAGAGTGTTCAAAGCAGGCCC447580.208241357120621No Hit
AACTGTGGTAATTCTAGAGC446490.207734223023339No Hit
TTGGTGACTCTAGATAACCTCGGGCCG430690.20038310491594857No Hit
ACGATGCCGACCGGCGATGCGGCGGCG429750.1999457599146228No Hit
CCGGGGTCCGGTGCGGAGTGCCCT419670.1952559326663636No Hit
AACGATGCCGACCGGCGATGCGGCGGCG417950.1944556843660654No Hit
AGCTAGGAATAATGGAAT409470.1905102741413394No Hit
AAAGATTAAGCCATGCATGTCTA406960.18934246993567166No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAC402130.1870952610458808No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTC392030.1823961285848274No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATA391600.18219606650975287No Hit
TAGGAATAATGGAAT381830.1776504700598032No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA380690.1771200729305358No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC376090.17497987398787782No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG375150.17454252898655206No Hit
AAGGGAGTCGGGTTCAGATCCCCGAATCCGGAG359930.1674612620501924No Hit
TTCTAGAGCT351990.16376709257090885No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGG345950.160956918307071No Hit
TTTGGTGACTCTAGATAACCTCGG332480.1546898574902008No Hit
TGGTGACTCTAGATAACCTC327090.1521821026421733No Hit
TTTGGTGACTCTAGATAACCTCGGGCCG317470.14770629528817988No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCT315220.1466594588488363No Hit
CCTGCGGAAGGATCATT309550.1440214310216905No Hit
AAATTAGAGTGTTCAAAGCAGGCCC306330.1425232917618299No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCG292040.13587471722039893No Hit
TTGGTGACTCTAGATAACC285740.13294357519023692No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATA284910.13255740885927908No Hit
TCGGGCCGATCGCA282190.13129189991927262No Hit
ACTGTGGTAATTCTAGAGC281400.1309243440139031No Hit
AGGGCGCACCACCGGCCCGTCTCG277500.1291098275190409No Hit
ATAACCTCGGGCCGATCGCAC276490.12863991427293556No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGA273400.12720225889623707No Hit
GCAGCTAGGAATAATGGAAT272410.12674165086292588No Hit
ATAACCTCGGGCCGATCGCA271200.1261786854888789No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAA269010.1251597646879178No Hit
TCGGGCCGATCGCAC267040.12424320122769254No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG255020.11865076833839933No Hit
TTTTGGTGACTCTAGATAACCTC251890.11719450253611248No Hit
CAGCTAGGAATAATGGAAT250680.11663153716206551No Hit
AGGGCGCACCACCGGCCCGTCTCGC244440.11372831077028599No Hit
TAGATAACCTCGGGCCGATCGCAC242030.1126070326285891No Hit
TAGATAACCTCGGGCCGATCGCA238430.11093209432563936No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGAT237910.11069015879299107No Hit
AGATAACCTCGGGCCGATCGCAC235250.10945256549136712No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCG233170.10848482336077395No Hit
CACTCGCCGAATCCCGGGGCCGAGGG232760.10829406649849355No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG232480.10816379351937525No Hit
CCGCAGCTAGGAATAATGGAAT232260.10806143617863943No Hit
CGAGGGCGCACCACCGGCCCGTCTCG227770.10597241590634937No Hit
CGGTTGGCCTCGGATAGCCGGTC226270.10527452494678699No Hit
TTGGTGACTCTAGATAACCTCGGGCCGA226040.10516751499965407No Hit
ATACCGCAGCTAGGAATAATGGAAT225060.10471155957273999No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG221970.10327390419604147No Hit
AGATAACCTCGGGCCGATCGCA220670.10266906536442073No Hit
GTTGGCCTCGGATAGCCGGTC217070.10099412706147103No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)