FastQCFastQC Report
Fri 8 Sept 2023
SRR14510087_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14510087_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20382533
Total Bases511.9 Mbp
Sequences flagged as poor quality0
Sequence length10-86
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGTGACTCTAGATAACCTCG6615173.2455092799310075No Hit
AACTGTGGTAATTCTAGAGCT5110192.5071417767359923No Hit
TTGGTGACTCTAGATAACCTC4503382.2094309868160154No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCG4259502.0897795185711217No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATT3579521.7561703444807377No Hit
TTGGTGACTCTAGATAACCT3292591.6153978506989293No Hit
ACTGTGGTAATTCTAGAGCT2931701.4383393859830866No Hit
GAGGGCGCACCACCGGCCCGTCTCG2804201.3757858260305527No Hit
GAGGGCGCACCACCGGCCCGTCTCGC2128601.0443255507055969No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATT1971030.9670191629273948No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCA1711440.8396601148640357No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1682950.8256824605656226No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCA1664890.8168219327794048No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCAC1473210.7227806279033131No Hit
GGTTGGCCTCGGATAGCCGGTC1147170.562820136241163No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC1114610.5468456741858335No Hit
TTTGGTGACTCTAGATAACCTCG1101460.5403940717279839No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAT1077290.5285358792256095No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT931180.45685195260078815No Hit
TAACTGTGGTAATTCTAGAGCT906160.4445767363653968No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCC879050.4312761323629404No Hit
CTGTGGTAATTCTAGAGCT875070.42932348006010834No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCA875030.4293038554138487No Hit
AGGGAGTCGGGTTCAGATCCCCGAATCCGGAG847460.41577756797940674No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCAC833480.40891875411167No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGAT780490.38292100397924045No Hit
TTGGTGACTCTAGATAACCTCGG753920.36988533270128887No Hit
CACTCGCCGAATCCCGGGGCCGAGGGA736490.36133389309366015No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGC732830.35953823796090506No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGC727410.35687909839272675No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCG712580.3496032607919732No Hit
TTTGGTGACTCTAGATAACCTC707220.3469735581931843No Hit
GTTGGCCTCGGATAGCCGGTC677590.33243660147637194No Hit
AGGGCGCACCACCGGCCCGTCTCG642950.31544165781554234No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAG589500.28921822425112714No Hit
CACTCGCCGAATCCCGGGGCCGAGGG562030.2757409984323342No Hit
AACTGTGGTAATTCTAGAGCTA548610.2691569296122322No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC542230.26602679853382305No Hit
ATTGATCATCGACACTTCGAACG514490.25241710635277764No Hit
AATTAGAGTGTTCAAAGCAGGCCC510870.2506410758662822No Hit
AAAGATTAAGCCATGCATGTCTA505020.24777097135081297No Hit
AAGGGAGTCGGGTTCAGATCCCCGAATCCGGAG491540.24115746556132153No Hit
AACTGTGGTAATTCTAGAGC481660.2363101779351958No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGA469920.23055034425799778No Hit
TGGTGACTCTAGATAACCTCG438270.21502234290507466No Hit
AGGGAGTCGGGTTCAGATCCCCGAATCCGG435870.21384486412949752No Hit
CCTGCGGAAGGATCATTA435110.21347199585056478No Hit
AGCTAGGAATAATGGAAT427400.20968934528402336No Hit
TTTGGTGACTCTAGATAACCT424070.20805559348291008No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGAT419920.20601953643347468No Hit
AGGGCGCACCACCGGCCCGTCTCGC417760.20495980553545526No Hit
GAGGGCGCACCACCGGCCCGTCTC415200.20370382817483967No Hit
CCGGGGTCCGGTGCGGAGTGCCCT415030.20362042342823633No Hit
CGAGGGCGCACCACCGGCCCGTCTCG414740.2034781447428541No Hit
ACGGTGTGAGGCCGGTAGCGGCCCCCG402200.19732581814046368No Hit
AACGATGCCGACCGGCGATGCGGCGGCG399610.1960551222951534No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCA391680.19216453617418405No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGC377300.1851094758438512No Hit
AGGGAGTCGGGTTCAGATCCCCGAATCCGGA368140.18061543185039858No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGG363140.17816235106794626No Hit
ACGATGCCGACCGGCGATGCGGCGGCG361050.17713696330088122No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGG357940.17561114705419587No Hit
CCTGCGGAAGGATCATT355700.17451216686365725No Hit
AAATTAGAGTGTTCAAAGCAGGCCC355580.17445329292487838No Hit
TTGGTGACTCTAGATAACCTCGGGCCG349190.17131825568490433No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAA336150.1649206210042687No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG335070.164390755555259No Hit
CGGTTGGCCTCGGATAGCCGGTC333970.1638510777831195No Hit
ATTGATCATCGACACTTCGAACGC324090.15900379015699373No Hit
ACTGTGGTAATTCTAGAGC319440.1567224250293131No Hit
TGGTGACTCTAGATAACCTC314820.15445577838632715No Hit
TTGGTGACTCTAGATAACC303240.1487744432941676No Hit
ATTGATCATCGACACTTCGAACGCA301840.14808758067508096No Hit
GTTGAACCCCATTCGTGATGGGGATCGGG300200.1472829701784366No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTT292740.14362297365101775No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAA291120.1428281754775032No Hit
AGGGAGTCGGGTTCAGATCCCCGAATCCGGAGT289290.1419303479111257No Hit
AAGGGAGTCGGGTTCAGATCCCCGAATCCGGA284980.13981579227665178No Hit
TTCTAGAGCT282300.13850094097725735No Hit
AGGAATAATGGAAT281450.13808391724424046No Hit
AAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGT278790.13677887826797583No Hit
ATTGATCATCGACACTTCGAAC276730.13576820898560546No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGC275620.13522362505190105No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAC271350.13312869406368677No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG271070.13299132153986945No Hit
CGAGGGCGCACCACCGGCCCGTCTCGC270940.1329275414395257No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTG270820.13286866750074686No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTCG268940.1319463091265448No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAA265500.1302585895482176No Hit
AAGGGAGTCGGGTTCAGATCCCCGAATCCGG258370.1267604963524406No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCC257890.12652500059732516No Hit
TTGGTGACTCTAGATAACCTCGGGCCGA252230.12374811315158916No Hit
AGATTAAGCCATGCATGTCTA251990.12363036527403146No Hit
CAGCTAGGAATAATGGAAT249650.12248232346784375No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATT248130.12173658690997825No Hit
TGGTGACTCTAGATAACCT247730.12154034044738209No Hit
ATTGATCATCGACACTTCGAA247460.12140787408512965No Hit
AAGATTAAGCCATGCATGTCTA241390.11842983401523255No Hit
ACTCGCCGAATCCCGGGGCCGAGGGA237060.11630546605762886No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGG236960.1162564044419798No Hit
CGGCCGAGGTGGGATCCCG232880.11425469052349871No Hit
TTTGGTGACTCTAGATAACCTCGGGCCG232500.11406825638403234No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCG227090.11141402297741895No Hit
TTTTGGTGACTCTAGATAACCTCG227080.11140911681585405No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC225410.11058978783451497No Hit
TTTGGTGACTCTAGATAACCTCGG219940.10790611745851214No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCT217900.10690526049927161No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCG214300.10513904233590596No Hit
TAGGAATAATGGAAT212230.1041234668919707No Hit
ACTCGCCGAATCCCGGGGCCGAGGG208390.10223950085104731No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCT207110.10161151217073952No Hit
TGCATTTATCAGTTGGTGACTCTAGATAACCTC204880.10051743814176581No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)