FastQCFastQC Report
Thu 14 Sept 2023
SRR14525704_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14525704_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119650833
Total Bases14.9 Gbp
Sequences flagged as poor quality0
Sequence length121-129
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCT92866907.761492141053459Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAA69044605.7705072558918165Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA54245144.533619920556675No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTC44995703.7605839317474707No Hit
AGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAACTCCAG40576183.3912158388400018Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC23330191.949856044880189No Hit
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCAGATCGGAAGAGCACAC17671001.4768806498823122No Hit
AAGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGA17223981.439520274798254Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC10948680.9150525512847871No Hit
TCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCTG10615350.8871939905341069Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC9530610.7965351983801066Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA9149570.7646892019548246No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTCT8466910.7076348561651886Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG8414590.7032621327425276TruSeq Adapter, Index 7 (100% over 49bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT8073890.6747876130540604No Hit
TCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCAGATCGGAAGAGCACACG7622970.6371012895497351No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC7390410.6176647345196502No Hit
ACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTCC6626280.5538014098071511Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACG6215700.5194865630396405No Hit
AACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGTCT5939160.49637431274715826Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTAGATCGGAAGAGCACAC5878620.49131459034639563No Hit
AAGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGA5333980.4457954755734964Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCA5222370.4364675003975944No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGAGATCGGAAGAGCACACGTC4974740.4157714472409899No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTCTG4953580.4140029681197456Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTCCGTAGCGGTCCTGACGTGCAGATCGGAAGAGCACACGTCTGAACT4892350.4088855779215511Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCTACCATCTGTGGGATTATGACTAAGATCGGAAGAGCACACGTCTGAA4808360.40186598617328473Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTC4087810.34164492611597613No Hit
ACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGTCTG4079390.3409412118342712Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAGGGCAGGGCGCCCTGGAATGGGAGATCGGAAGAGCACACGTCTGAACT3895620.32558235511824646Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC3874790.32384145624794775Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGAA3573330.2986464791264763Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGGGCTTGGCGGAATCAGCGGGGAGATCGGAAGAGCACACGTCTGAAC3351340.2800933278918334Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTGGGGCGGTACACCTGTCAGATCGGAAGAGCACACGTCTGAACTCCAG3248680.2715133625521855Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACG3116220.2604428169756244No Hit
AGGTCCGTAGCGGTCCTGACGTGCAAGATCGGAAGAGCACACGTCTGAAC3073090.2568381617535417Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTC2991590.25002667553513813No Hit
AAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGA2755520.23029676692681278Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT2616610.2186871528090406Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTAGATCGGAAGAGCACACG2545610.21275322002981792No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATG2488230.20795759942598976TruSeq Adapter, Index 7 (97% over 49bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTCT2472940.20667971446550648Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGCGAAGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACA2461710.20574115016817307No Hit
TCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG2458810.20549877826592317Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATCTCGCGCCGGGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC2457000.20534750476831198Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGAA2447540.20455687090786906Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAACTGGTGCGGACCAGGGGAATCCGAGATCGGAAGAGCACACGTCTGA2437880.2037495217438227Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGGGCTTGGCGGAATCAGCGGGGAGATCGGAAGAGCACACGTCTGAACT2384210.19926397002183846Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCTAGATCGGAAGAGCACACGTCT2372710.1983028400646404Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAA2324370.19426275118368794Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTACCATCTGTGGGATTATGACTAGATCGGAAGAGCACACGTCTGAAC2282110.1907308075322802Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTC2277570.190351370140482Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTT2267710.1895273056728322No Hit
TCCGGCGGCGTCCGGTGAGCTCTCGAGATCGGAAGAGCACACGTCTGAAC2219970.18553736270269008Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGAGATCGGAAG2205870.18435893379864726No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC2130240.17803804174100485No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGC1985890.16597377136521899No Hit
AGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGAA1973450.16493407948108477Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTC1961890.1639679349328057Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCGAAGATCGGAAGAGCACACGTC1863370.15573397637774908No Hit
AGGTCCGTAGCGGTCCTGACGTGAGATCGGAAGAGCACACGTCTGAACTC1855440.15507121458987252Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG1850310.15464246705244417Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCA1652960.1381486412217456No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGAGATCGGAAGAGCACACGTCTGA1587550.1326819011782392Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCAAGATCGGAAGAGCACAC1575260.13165474577180755No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACAGATCGGAAGAGCAC1568230.13106720285014647No Hit
TGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACACGTCT1542790.12894101623178836Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTGGTGCGGACCAGGGGAATCCGAGATCGGAAGAGCACACGTCTGAAC1541010.1287922500297177Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTC1534390.12823897348044372TruSeq Adapter, Index 7 (100% over 50bp)
AGCCGACTTAGAACTGGTGCGGACCAGGGGAAGATCGGAAGAGCACACGT1533180.12813784589364288No Hit
AGACCCTGTTGAGCTTGACTCAGATCGGAAGAGCACACGTCTGAACTCCA1499380.12531295958466082Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCCTACTTGGATAACTGTGGTAATTCTAAGATCGGAAGAGCACACGTCTG1491600.12466273427448682Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGT1403130.1172687197255033No Hit
TCTCGGCGCCCCCTCGATGCTTAGATCGGAAGAGCACACGTCTGAACTCC1399470.11696282966956026Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGTCT1399150.11693608518379475Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGGATTTTTGGAAATAGGAGATGGAAGATCGGAAGAGCACACGTCTGA1392000.11633851307997162Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGT1368750.11439535903607125No Hit
AGCCGACTTAGAACTGGTGCGGACCAGATCGGAAGAGCACACGTCTGAAC1361620.11379945846260846Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGCGGGTGTTGACGCGATGTGAGATCGGAAGAGCACACGTCTGAACTCC1350210.11284585039203195Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGA1307680.10929134108075955Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAACTCCA1296120.10832519653248049Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAAATCTCGCGCCGGGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGT1271940.10630431632682408Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGTCTAACACGTGCGCGAGTCGGGGGCTCGAGATCGGAAGAGCACACGTC1253890.10479576017661324No Hit
AACGGGCTTGGCGGAATCAGCGGGGAAGATCGGAAGAGCACACGTCTGAA1246120.10414637063161941Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGATCGGAAGAG1243010.10388644766058587No Hit
AGAGGGTGTAAATCTCGCGCCGGGCCGAGATCGGAAGAGCACACGTCTGA1213920.1014552067514649Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGCTACCATCTGTGGGATTATGACTAAGATCGGAAGAGCACACGTCTGA1206760.1008567988824616Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGGCGTGAAACCGTTAAGAGGAGATCGGAAGAGCACACGTCTGAACTCC1206570.10084091934403833Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCATTCGTAGACGACCTGCAGATCGGAAGAGCACACGTCTGAACTCCAG1206340.10082169674489438Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)