FastQCFastQC Report
Thu 14 Sept 2023
SRR14525705_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14525705_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112457172
Total Bases14.1 Gbp
Sequences flagged as poor quality0
Sequence length121-129
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAA77593246.899803598120002Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCT71103306.322700343202654Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAACTCCAG40565953.6072354727184495Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTC33393252.9694193270305607No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA31462322.797715738396836No Hit
AAGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGA18301891.627454227641435Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC12263271.0904835842750875No Hit
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCAGATCGGAAGAGCACAC11769461.0465726454511945No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG11447431.0179368551078272TruSeq Adapter, Index 9 (100% over 49bp)
ACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTCC9141100.8128516694337645Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGAA8981770.7986836090809752Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCTG8860360.7878874990738697Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC8810990.7834973833416334Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGA8464520.7526883212037379Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTCT7261800.6457391619273514Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT7137230.6346620560581053No Hit
AAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGA6829080.607260513362367Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC6765850.6016379284373254No Hit
AATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC6230240.5540100190319565Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCTACCATCTGTGGGATTATGACTAAGATCGGAAGAGCACACGTCTGAA6141780.5461439133468516Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA5711060.5078431102642347No Hit
AACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGTCT5614580.4992638441948371Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCAGATCGGAAGAGCACACG5455670.485133131393345No Hit
AGGTCCGTAGCGGTCCTGACGTGCAGATCGGAAGAGCACACGTCTGAACT5157230.45859502851449974Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTGGGGCGGTACACCTGTCAGATCGGAAGAGCACACGTCTGAACTCCAG4832140.4296871345831104Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTT4590270.40817939117302365No Hit
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTAGATCGGAAGAGCACAC4547850.40440728849201363No Hit
AATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGAA4116040.3660095596215064Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCGACTTAGAACTGGTGCGGACCAGGGGAGATCGGAAGAGCACACGTC3912600.34791911715510687No Hit
ACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGTCTG3850060.34235788892148206Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGGATTTTTGGAAATAGGAGATGGAAGATCGGAAGAGCACACGTCTGA3649670.32453866081569255Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTC3530220.3139168393813069Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGTCCGTAGCGGTCCTGACGTGCAAGATCGGAAGAGCACACGTCTGAAC3453490.3070937974502862Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGGGCAGGGCGCCCTGGAATGGGAGATCGGAAGAGCACACGTCTGAACT3433240.30529311194131753Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTC3394460.301844688038216No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTCTG3322820.2954742628598201Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCGCGTATG3124090.27780264650439546TruSeq Adapter, Index 9 (97% over 49bp)
AGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGAA2856100.253972241094592Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTC2819840.2507479024992732Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCA2817140.2505078110980774No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC2772180.24650984465446102No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACG2767660.24610791386431094No Hit
TCCTACTTGGATAACTGTGGTAATTCTAAGATCGGAAGAGCACACGTCTG2699560.24005227518970512Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG2624300.2333599496882244Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT2591450.23043883764034184Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG2568770.22842206987029692Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATGGATTTTTGGAAATAGGAGATGGAAGATCGGAAGAGCACACGTCTG2377560.2114191525285733Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAA2329570.20715175017917042Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGA2280320.20277230517587622Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAACTGGTGCGGACCAGGGGAATCCGAGATCGGAAGAGCACACGTCTGA2239400.19913358660664168Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCTAGATCGGAAGAGCACACGTCT2235880.19882057855767526Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGGGCTTGGCGGAATCAGCGGGGAGATCGGAAGAGCACACGTCTGAAC2197700.19542550829928393Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTC2178190.19369062561879113No Hit
TCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTAGATCGGAAGAGCACACG2104450.18713346268390957No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGAGATCGGAAG1993980.17731016746535294No Hit
AATGGATTTTTGGAAATAGGAGATGAGATCGGAAGAGCACACGTCTGAAC1987210.1767081605075397Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTACCATCTGTGGGATTATGACTAGATCGGAAGAGCACACGTCTGAAC1888480.167928818270479Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACG1857780.16519889011614128No Hit
ACGGGCTTGGCGGAATCAGCGGGGAGATCGGAAGAGCACACGTCTGAACT1806490.16063804272083243Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTGA1801930.16023255502103503Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACACGTCT1790780.15924106645683744Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG1721600.15308939122175327Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCGGCGGCGTCCGGTGAGCTCTCGAGATCGGAAGAGCACACGTCTGAAC1715620.15255763322947513Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTACCATCTGTGGGATTATGACTTAGATCGGAAGAGCACACGTCTGAA1653740.14705509400503153Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGAAC1644140.1462014356896686Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTCT1640690.14589465223258505Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATCTCGCGCCGGGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1615450.14365024224510997Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCTCGGCGCCCCCTCGATGCTTAGATCGGAAGAGCACACGTCTGAACTCC1573020.13987725033668819Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCGAAGATCGGAAGAGCACACGTC1542130.13713042686152555No Hit
CGGCGGGTGTTGACGCGATGTGAGATCGGAAGAGCACACGTCTGAACTCC1486170.1321543102648891Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGGGCGAAGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACA1485950.1321347472618287No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGTCT1468830.1306123899327648Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGGTCGTCCGACCTGGGTATAGGGGAGATCGGAAGAGCACACGTCTGA1466650.13041853835698447Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGCTACCATCTGTGGGATTATGACTAAGATCGGAAGAGCACACGTCTGA1454660.12935235469019263Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGACGTGGCGACCCGCTGAATTTAAGCAGATCGGAAGAGCACACGTCTGA1453830.12927854881501022Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC1439550.12800873207090785No Hit
AGGTCCGTAGCGGTCCTGACGTGAGATCGGAAGAGCACACGTCTGAACTC1436080.12770017015900062Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACTGGTGCGGACCAGGGGAATCCGAGATCGGAAGAGCACACGTCTGAAC1357010.12066904901361025Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGC1310460.11652969541151187No Hit
AGGTCCGTAGCGGTCCTGACGTGTAGATCGGAAGAGCACACGTCTGAACT1305030.11604684492688469Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGACCCTGTTGAGCTTGACTCAGATCGGAAGAGCACACGTCTGAACTCCA1285880.114343974433218Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCCGACTTAGAACTGGTGCGGACCAGATCGGAAGAGCACACGTCTGAAC1272470.11315152047394542Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCATTCGTAGACGACCTGCAGATCGGAAGAGCACACGTCTGAACTCCAG1241450.11039313704242892Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCAAGATCGGAAGAGCACAC1206210.10725949964311746No Hit
CAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAACTCCA1199560.10666816341424626Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)