FastQCFastQC Report
Thu 14 Sept 2023
SRR14525706_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14525706_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122829483
Total Bases15.4 Gbp
Sequences flagged as poor quality0
Sequence length121-129
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA56469074.5973546921141075No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCT50039044.0738622990051985Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAA37418583.046384230079353Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAACTCCAG33645852.7392324039986393Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTC22292691.8149298894305368No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC18864191.5358030937897866No Hit
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC17576261.4309479752511862Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC13897961.1314840428010269Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCAGATCGGAAGAGCACAC13223221.0765509775857316No Hit
AAGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGA10058540.8189027385224766Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGAA10012690.8151699213779154Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGA9136020.7437969921276962Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC8351630.6799369170999442No Hit
AAGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGA7246740.5899837582154441Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTC6521960.5309767525440126Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA6188590.5038358746490856No Hit
AATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGAA5965850.4857017919712322Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG5762720.46916423152249204TruSeq Adapter, Index 8 (100% over 49bp)
TCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCAGATCGGAAGAGCACACG5734640.4668781354391926No Hit
AGGTCCGTAGCGGTCCTGACGTGCAGATCGGAAGAGCACACGTCTGAACT5480000.44614695642739133Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGGGCAGGGCGCCCTGGAATGGGAGATCGGAAGAGCACACGTCTGAACT5479030.44606798515955653Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCTG5273410.42932770465214776Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT5152960.41952142711534496No Hit
AATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG4986820.40599535862248964Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTCT4959760.403792304490934Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTCC4890790.3981772031068469Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT4774370.3886990226931103Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTAGATCGGAAGAGCACAC4449240.36222899350638804No Hit
AACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGTCT4238690.345087343565551Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC4011600.32659911138761366No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGAGATCGGAAGAGCACACGTC3832030.3119796571967986No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGA3753670.3056000813745996Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG3619160.2946491275225835Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAATCTCGCGCCGGGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC3529160.2873218964863672Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AACGGGCTTGGCGGAATCAGCGGGGAGATCGGAAGAGCACACGTCTGAAC3496040.28462547546503963Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGGATTTTTGGAAATAGGAGATGGAAGATCGGAAGAGCACACGTCTGA3375750.2748322241167456Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGGGGCGGTACACCTGTCAGATCGGAAGAGCACACGTCTGAACTCCAG3194210.2600523849799156Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCA3193650.2600067933201347No Hit
TCCTACTTGGATAACTGTGGTAATTCTAAGATCGGAAGAGCACACGTCTG3168130.2579291162529765Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGTCTG3138210.2554932190018255Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACG2944050.23968593924636158No Hit
TGCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAA2801630.2280910031999402Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCTACCATCTGTGGGATTATGACTAAGATCGGAAGAGCACACGTCTGAA2795870.22762206041362235Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACG2789350.2270912432318876No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTCTG2755660.22434841641399728Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC2683580.21848011849076987No Hit
ACGGGCTTGGCGGAATCAGCGGGGAGATCGGAAGAGCACACGTCTGAACT2647910.21557609259008279Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTTTG2618240.21316054875847681TruSeq Adapter, Index 8 (97% over 49bp)
AGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGAA2604480.21204029654671752Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTCGGCGCCCCCTCGATGCTTAGATCGGAAGAGCACACGTCTGAACTCC2468960.2010071148797394Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGTCCGTAGCGGTCCTGACGTGAGATCGGAAGAGCACACGTCTGAACTC2404990.19579908188655323Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAATGGATTTTTGGAAATAGGAGATGGAAGATCGGAAGAGCACACGTCTG2356280.19183342162239664Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTT2350420.19135633746826078No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTC2194010.17862242406409867No Hit
AGGTCCGTAGCGGTCCTGACGTGCAAGATCGGAAGAGCACACGTCTGAAC2135350.17384669770204927Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGATG2105310.17140103080951663TruSeq Adapter, Index 8 (97% over 49bp)
AGAACTGGTGCGGACCAGGGGAATCCGAGATCGGAAGAGCACACGTCTGA2072220.16870705219853444Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTC2046840.16664077304632147No Hit
TCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTAGATCGGAAGAGCACACG1928580.15701279146473326No Hit
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCA1851650.15074963720233195Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGAAC1812080.1475280979567422Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGTCT1805540.1469956525014438Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTCTAACACGTGCGCGAGTCGGGGGCTCGAGATCGGAAGAGCACACGTC1783570.14520699399182524No Hit
AATGGATTTTTGGAAATAGGAGATGAGATCGGAAGAGCACACGTCTGAAC1747410.1422630753888299Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTGA1732830.14107606396096287Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACACGTCT1725050.14044266554472104Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCCA1688490.13746618147045364Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGAGATCGGAAGAGCACACG1627920.1325349549830801No Hit
ACGTCTCGTCGCGCGCGCGTCCGAGATCGGAAGAGCACACGTCTGAACTC1574290.12816873942227697Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGGCGGCGTCCGGTGAGCTCTCGAGATCGGAAGAGCACACGTCTGAAC1556270.12670166494147012Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGGTTG1499040.12204236013921836TruSeq Adapter, Index 8 (97% over 45bp)
TGGGGCGAAGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACA1480900.12056551601702989No Hit
TGGGCGCGCGCCGCGGCTGGACGAGATCGGAAGAGCACACGTCTGAACTC1469570.11964309904324844Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAGATCGGAAGAGC1443180.11749459207607345No Hit
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG1384310.11270176884160621Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGAGATCGGAAG1382500.1125544100841001No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCTAGATCGGAAGAGCACACGTCT1381300.11245671367028387Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACAGATCGGAAGAGCAC1366190.11122655299298134No Hit
AGGGCGTGAAACCGTTAAGAGGAGATCGGAAGAGCACACGTCTGAACTCC1356080.11040346070657969Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTC1341950.10925308543389374Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAAATCTCGCGCCGGGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGT1302390.10603236032508578Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCAAGATCGGAAGAGCACAC1280840.10427789556030291No Hit
AATGGATTTTTGGAAATAGGAGATCGGAAGAGCACACGTCTGAACTCCAG1255410.10220754572418089Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTCT1237390.10074047124337404Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACTGGTGCGGACCAGGGGAATCCGAGATCGGAAGAGCACACGTCTGAAC1233440.10041888721456231Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)