FastQCFastQC Report
Thu 14 Sept 2023
SRR14525707_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14525707_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129998813
Total Bases16.2 Gbp
Sequences flagged as poor quality0
Sequence length121-129
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCT63933904.918037213155169Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAA59683854.5911073049567Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAACTCCAG38675862.975093318736687Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA25753881.9810857811447862No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTC25417731.9552278527343168No Hit
AATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCC20064401.543429477313766Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC19365981.4897043713776064Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGCTACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGA15613121.2010201970074912Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGAA14376051.1058600973533503Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCAGATCGGAAGAGCACAC11817470.909044454121285No Hit
AAATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGA10765900.8281537155266179Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG10522880.8094596986820181TruSeq Adapter, Index 10 (100% over 49bp)
ACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTCC9784580.7526668724275198Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTC9586410.7374228870843613Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC8650910.6654606915526221No Hit
AAGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGA7636690.587443056114674Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGTCGTCCGACCTGGGTATAGGGGCGAGATCGGAAGAGCACACGTCTG7520930.578538359423328Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGAA6996330.5381841448044606Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACGTCTGAACTCCAGT6832890.5256117223162646Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGGTCCGTAGCGGTCCTGACGTGCAGATCGGAAGAGCACACGTCTGAACT6443820.4956829875054321Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAG5993490.46104190197490497Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCAGATCGGAAGAGCACACG5696370.4381863086703722No Hit
AAGGGCAGGGCGCCCTGGAATGGGAGATCGGAAGAGCACACGTCTGAACT5334730.41036759312563875Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCTACTTGGATAACTGTGGTAATTCTAAGATCGGAAGAGCACACGTCTG5170830.39775978569896636Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTCT4794670.3688241368788498Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATCTCGCGCCGGGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC4724450.36342254909665983Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGAACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGT4603380.3541093871372502No Hit
ACTGGGGCGGTACACCTGTCAGATCGGAAGAGCACACGTCTGAACTCCAG4574960.3519232133296479Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGTCT4456370.3428008223428932Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGGGCTTGGCGGAATCAGCGGGGAGATCGGAAGAGCACACGTCTGAAC4331010.33315765737030234Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGGATTTTTGGAAATAGGAGATGGAAGATCGGAAGAGCACACGTCTGA4177930.3213821652356164Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAACTCCAG4072690.3132867067024681Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTCTG4062330.3124897763489579Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCAC3865180.2973242532606817No Hit
AGGTCCGTAGCGGTCCTGACGTGAGATCGGAAGAGCACACGTCTGAACTC3863130.29716655951312415Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAATGGATTTTTGGAGCAGGGAGATGGAGATCGGAAGAGCACACGTCTGA3693720.28413490206252884Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCGACTTAGAACTGGTGCGGACCAGGGGAGATCGGAAGAGCACACGTC3520600.270817857390744No Hit
ACTGGTGCGGACCAGGGGAATCCGACTGAGATCGGAAGAGCACACGTCTG3298690.2537477015270901Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGGCTTGGCGGAATCAGCGGGGAGATCGGAAGAGCACACGTCTGAACT3292180.25324692772387086Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCGCGTATG3267290.25133229485718456TruSeq Adapter, Index 10 (97% over 49bp)
AGCTACCATCTGTGGGATTATGACTAAGATCGGAAGAGCACACGTCTGAA3207750.24675225303787968Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATGGATTTTTGGAAATAGGAGATGAGATCGGAAGAGCACACGTCTGAAC3195830.24583532158866714Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGCCTCTCGGCGCCCCCTCGATGCTCAGATCGGAAGAGCACACGTCTGAA3140120.24154989784406725Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGTGGATTTAGCAGTAAACTGAGAGAGATCGGAAGAGCACACGTCTGAA3014950.2319213483895426Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTAGATCGGAAGAGCACAC2926920.22514974809808458No Hit
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACG2893420.22257280149165667No Hit
ATGGATTTTTGGAAATAGGAGATGGAGATCGGAAGAGCACACGTCTGAAC2884210.2218643334843373Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA2705200.20809420775249693No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGTC2573560.19796796144592488No Hit
TGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTT2530180.19463100790004906No Hit
TCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGAGATCGGAAGAGCACACG2522380.19403100242153748No Hit
TACCATCTGTGGGATTATGACTGAGATCGGAAGAGCACACGTCTGAACTC2417570.18596862111348664Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAATGGATTTTTGGAAATAGGAGATGGAAGATCGGAAGAGCACACGTCTG2402470.1848070720461117Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAGATCGGAAGAGCACACG2401360.18472168665109273No Hit
AGGTCCGTAGCGGTCCTGACGTGCAAGATCGGAAGAGCACACGTCTGAAC2323890.17876240146900418Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGGATTTTTGGAGCAGGGAGAGATCGGAAGAGCACACGTCTGAACTCCA2303500.17719392560915154Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAATGGATTTTTGGAGCAGGGAGATCGGAAGAGCACACGTCTGAACTCCA2196950.168997696925125Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCTCTCGGGGCCACGCGCGCGTCCCCCGAGATCGGAAGAGCACACGTCT2108370.16218378855505397Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC2081780.16013838526356391No Hit
AGTCTAACACGTGCGCGAGTCGGGGGCTCGAGATCGGAAGAGCACACGTC1976560.15204446520600154No Hit
AGAACTGGTGCGGACCAGGGGAATCCGAGATCGGAAGAGCACACGTCTGA1966040.15123522704780387Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCAAGATCGGAAGAGCACACGTC1893770.14567594551805638No Hit
TCTCGGCGCCCCCTCGATGCTTAGATCGGAAGAGCACACGTCTGAACTCC1879280.1445613199560522Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGTCTCGTCGCGCGCGCGTCCGAGATCGGAAGAGCACACGTCTGAACTC1870140.14385823661328354Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTC1869080.1437766974072294TruSeq Adapter, Index 10 (100% over 50bp)
AATGGATTTTTGGAGCAGGGAGATGGAAGATCGGAAGAGCACACGTCTGA1861900.1432243846718816Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGGCGGCGTCCGGTGAGCTCTCGAGATCGGAAGAGCACACGTCTGAAC1848130.1421651442309708Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGCGCGCGCCGCGGCTGGACGAGATCGGAAGAGCACACGTCTGAACTC1839580.14150744591798695Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATGGATTTTTGGAAATAGGAGATCGGAAGAGCACACGTCTGAACTCCAG1805430.13888049885501647Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTC1788590.13758510241166588No Hit
AGCTACCATCTGTGGGATTATGACTAGATCGGAAGAGCACACGTCTGAAC1754610.13497123239117575Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGGCGTGAAACCGTTAAGAGGAGATCGGAAGAGCACACGTCTGAACTCC1747590.13443122746051536Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGGTCGTCCGACCTGGGTATAGGGGAGATCGGAAGAGCACACGTCTGA1669680.1284380958155364Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATCGGTCGTCCGACCTGGGTATAGGGGCAGATCGGAAGAGCACACGTCT1658440.12757347253624537Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGGATTTTTGGAAATAGGAGATGAGATCGGAAGAGCACACGTCTGAA1655730.12736500909435228Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCATCTGTGGGATTATGACTGAACGCCAGATCGGAAGAGCACACGTCTG1640960.12622884487414512Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATCTCGCGCCGGGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1633960.12569037841907066Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCGACCTCAGATCAGACGTAGATCGGAAGAGCACACGTCTGAACTCCAG1559830.11998801865983193Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TGCGCGCTCGGTCGAAGAGGACGAGATCGGAAGAGCACACGTCTGAACTC1547600.1190472408390375Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCA1547360.1190287791320064No Hit
CGGCGGGTGTTGACGCGATGTGAGATCGGAAGAGCACACGTCTGAACTCC1534490.1180387700924623Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCATCTGTGGGATTATGACTGAACGCAGATCGGAAGAGCACACGTCTGA1486450.11434335173506546Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCTTAGTTGAACAGGGCCCTGAAGCGCGAGATCGGAAGAGCACACGTCT1466540.11281179928927505Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCTAGATCGGAAGAGCACACG1411650.10858945304369817No Hit
TAAATCTCGCGCCGGGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGT1380270.10617558484937858Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCAGATCGGAAGAGCACACGT1374920.10576404263014309No Hit
AATGGATTTTTGGAGCAGGGAGATGAGATCGGAAGAGCACACGTCTGAAC1367980.1052301916018264Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAATGGATTTTTGGAAATAGGAGAGATCGGAAGAGCACACGTCTGAACTC1343660.10335940528933907Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACTGGTGCGGACCAGGGGAATCCGAGATCGGAAGAGCACACGTCTGAAC1341370.10318324983475043Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGACCCTGTTGAGCTTGACTCAGATCGGAAGAGCACACGTCTGAACTCCA1321220.10163323568192888Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)