SRR14585402_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR14585402_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	82104009
Total Bases	12.3 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	57

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	5234524	6.375479180316273	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC	5183598	6.313452976455753	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAACC	2869436	3.4948792817169254	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAACA	2657473	3.236715274159146	Illumina PCR Primer Index 6 (96% over 29bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA	1720243	2.095199760586599	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACAC	1695131	2.0646141651865015	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAG	1575900	1.9193947033694787	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACAA	1369756	1.6683180476607422	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAGC	1359205	1.6554672744421042	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAGA	1287565	1.5682120954653993	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACCC	1250624	1.5232191646086368	Illumina PCR Primer Index 6 (96% over 29bp)
AGCGGGCCCCCGGTGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTT	1038017	1.264270786095232	TruSeq Adapter, Index 6 (95% over 22bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAACG	890095	1.0841066238312431	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACCA	882007	1.0742557041276755	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAC	789921	0.9620979652771888	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAATC	784630	0.9556537001743727	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATAC	759347	0.9248598323621444	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAA	735478	0.8957881703437892	Illumina PCR Primer Index 6 (96% over 29bp)
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA	663218	0.8077778516271963	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAT	648718	0.7901173254524028	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAATA	638698	0.777913292881959	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATAA	636150	0.7748099121444849	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGCC	568706	0.6926653240525685	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATCC	565902	0.68925014368056	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACGC	554376	0.6752118523225827	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAGG	533218	0.6494420997152526	Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	501112	0.6103380408622922	Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACAG	496385	0.6045807093293094	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGCA	450974	0.5492715952518226	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATCA	443175	0.5397726681044284	Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAC	429278	0.5228465762250415	Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAATT	418780	0.5100603552744909	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACGA	397721	0.48441118143207845	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACCG	392190	0.47767460417188645	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAACT	387749	0.47226561129310995	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATGC	386588	0.47085155123180406	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGGC	352745	0.4296318831398355	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAG	336321	0.4096279878367449	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAATG	326867	0.3981133247707795	Illumina PCR Primer Index 6 (96% over 29bp)
AGCGGGCCCCCGGTGGGCTGTAGGCACCATCAATATCTCGTATGCCGTCT	320889	0.3908323161174749	TruSeq Adapter, Index 6 (95% over 21bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACTC	316030	0.38491421289793537	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGGA	301897	0.36770068073046225	Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	272822	0.3322882808316948	Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATGA	269955	0.3287963685183753	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATAG	253967	0.309323506967851	Illumina PCR Primer Index 6 (96% over 29bp)
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA	241492	0.2941293646209164	Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATAAC	237180	0.2888774895267294	Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACAT	236791	0.288403700238316	Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAC	233733	0.28467915616641815	Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGCG	232361	0.28300810500008594	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAGT	224911	0.2739342484482092	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGTC	223045	0.27166152142461153	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATAT	214208	0.2608983441965666	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATCG	212291	0.2585635008395266	Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAAC	210647	0.2565611625615017	Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACGG	206671	0.25171852448764104	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACTA	203038	0.24729364920536342	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATTC	203020	0.24727172579356022	Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACAAC	202358	0.24646543142613173	Illumina PCR Primer Index 6 (100% over 28bp)
AGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAA	195453	0.23805536706496272	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGTA	191603	0.23336619287372437	Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAAA	190706	0.23227367618553194	Illumina PCR Primer Index 6 (100% over 28bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACA	190547	0.23208001938127043	Illumina PCR Primer Index 6 (100% over 28bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATAAA	190116	0.2315550754653162	Illumina PCR Primer Index 6 (100% over 28bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACC	186886	0.2276210410139656	Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATCT	184089	0.22421438641321398	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACCT	179471	0.2185898133183728	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGGG	178559	0.2174790271203444	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACGT	168072	0.2047062038103401	Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACAAA	164674	0.20056755084882638	Illumina PCR Primer Index 6 (100% over 28bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATACC	161739	0.19699281675758365	Illumina PCR Primer Index 6 (100% over 28bp)
GAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA	150612	0.18344049436124366	Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACACC	150126	0.18284856224255747	Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATTA	144783	0.17634096283897657	Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATCAC	143805	0.17514979079766982	Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATGG	143639	0.1749476082221515	Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACCCC	137400	0.16734871009770058	Illumina PCR Primer Index 6 (100% over 28bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACCAC	135650	0.16521726728350136	Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACTG	133274	0.16232337692548	Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGACC	132999	0.1619884359118201	Illumina PCR Primer Index 6 (100% over 28bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAACC	132603	0.16150612085214985	Illumina PCR Primer Index 6 (100% over 28bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATACA	130646	0.1591225588022139	Illumina PCR Primer Index 6 (100% over 28bp)
TCTTCCGATCTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCT	129896	0.15820908331041422	Illumina PCR Primer Index 6 (96% over 27bp)
CGACGCTCTTCCGATCTCTGTAGGCACCATCAATATCTCGTATGCCGTCT	129802	0.15809459438210866	Illumina PCR Primer Index 6 (95% over 21bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGCAC	126435	0.15399369840758934	Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAT	124938	0.1521704013259572	Illumina PCR Primer Index 6 (96% over 29bp)
CTCTTCCGATCTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC	123751	0.1507246741142689	Illumina PCR Primer Index 6 (96% over 26bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAG	123538	0.15046524707459777	Illumina PCR Primer Index 6 (100% over 28bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAACA	120584	0.14686737160422947	Illumina PCR Primer Index 6 (100% over 28bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATCCC	118778	0.14466772261997582	Illumina PCR Primer Index 6 (100% over 28bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGACA	116563	0.1419699250008608	Illumina PCR Primer Index 6 (100% over 28bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACACA	115237	0.14035490033135897	Illumina PCR Primer Index 6 (100% over 28bp)
CGCTCTTCCGATCTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT	114735	0.13974348073551437	Illumina PCR Primer Index 6 (95% over 24bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGCT	109390	0.1332334453972887	Illumina PCR Primer Index 6 (96% over 29bp)
CACGACGCTCTTCCGATCTCTGTAGGCACCATCAATATCTCGTATGCCGT	109325	0.13315427752133274	No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACTT	104371	0.12712046740616528	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGTG	99834	0.12159454966443842	Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATCAA	99545	0.12124255710826495	Illumina PCR Primer Index 6 (100% over 28bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGCCC	97881	0.11921585948379207	Illumina PCR Primer Index 6 (100% over 28bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGCAA	95636	0.11648152284500506	Illumina PCR Primer Index 6 (100% over 28bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATTAC	94319	0.11487745988140483	Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATTG	94197	0.11472886786807207	Illumina PCR Primer Index 6 (96% over 29bp)
GACGCTCTTCCGATCTCTGTAGGCACCATCAATATCTCGTATGCCGTCTT	94119	0.11463386641692491	Illumina PCR Primer Index 6 (95% over 22bp)
ACGCTCTTCCGATCTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC	93684	0.11410405063168108	Illumina PCR Primer Index 6 (95% over 23bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAACAC	92124	0.11220402160873777	Illumina PCR Primer Index 6 (100% over 28bp)
GCTCTTCCGATCTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTG	91722	0.11171439874513313	Illumina PCR Primer Index 6 (96% over 25bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAAG	88253	0.10748927010372904	Illumina PCR Primer Index 6 (100% over 28bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATGAC	88066	0.10726151021444033	Illumina PCR Primer Index 6 (100% over 28bp)
CTTCCGATCTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTT	87970	0.10714458535148996	Illumina PCR Primer Index 6 (96% over 28bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACCAA	87691	0.10680477246854049	Illumina PCR Primer Index 6 (100% over 28bp)
CCGACGCTCTTCCGATCTCTGTAGGCACCATCAATATCTCGTATGCCGTC	86618	0.10549789353160575	No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGGAC	86570	0.10543943110013056	Illumina PCR Primer Index 6 (100% over 28bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGA	84251	0.10261496487948597	Illumina PCR Primer Index 6 (100% over 28bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATTCC	82984	0.10107180028200571	Illumina PCR Primer Index 6 (100% over 28bp)

Adapter Content

Adapter graph

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content