SRR14585406_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR14585406_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22785004
Total Bases	3.4 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	57

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	876477	3.846727435290334	Illumina PCR Primer Index 6 (96% over 29bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATATCT	748918	3.28688992110776	No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC	684755	3.0052880394491046	Illumina PCR Primer Index 6 (96% over 29bp)
CGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATATCTCGT	539350	2.3671270806009077	No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATATC	353259	1.5504013078075387	No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAACA	342600	1.5036205391932342	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAG	340836	1.4958786050684914	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAACC	323322	1.4190122591156884	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAT	312170	1.3700677866898774	Illumina PCR Primer Index 6 (96% over 29bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATATCTC	301855	1.3247967830069285	No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAATA	252073	1.1063109754117224	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACAC	233609	1.0252752204915128	Illumina PCR Primer Index 6 (96% over 29bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATAT	227804	0.9997979372748848	No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAGA	225437	0.989409525668725	Illumina PCR Primer Index 6 (96% over 29bp)
GTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATATCTCGTAT	215593	0.9462056710633011	No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAATC	209205	0.9181696873961488	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACAA	206025	0.9042131394841976	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATAC	205326	0.9011453322544951	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATAA	203258	0.8920691872601823	Illumina PCR Primer Index 6 (96% over 29bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA	189282	0.8307305980723111	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAA	181806	0.7979195439245919	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATAT	180142	0.790616494954313	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAGC	174561	0.7661223144836842	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAATG	173056	0.7595170929090028	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAGT	148727	0.6527407236794868	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACCC	147528	0.6474784906774649	Illumina PCR Primer Index 6 (96% over 29bp)
TCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATATCTCG	140338	0.6159226480715123	No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAGG	139505	0.6122667347348282	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAACG	137432	0.603168645482792	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAC	135129	0.5930611203754891	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATAG	129082	0.5665217350850585	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAATT	125643	0.5514284746230459	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATCC	120629	0.5294227729782273	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGTA	114991	0.5046784279695541	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAACT	114929	0.5044063191737864	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACCA	111174	0.4879261816236679	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATCA	108630	0.4767609432941069	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAG	103240	0.4531050334685041	Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	102160	0.4483650738002942	Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATGA	94892	0.4164669007738599	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATGC	94480	0.41465869393746874	Illumina PCR Primer Index 6 (96% over 29bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATA	94123	0.41309187393603264	No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATGT	90020	0.3950844160483799	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACAT	88514	0.388474805622154	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACAG	87717	0.38497689094107684	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAT	86852	0.38118053435496435	Illumina PCR Primer Index 6 (96% over 29bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCACCATCAAT	84900	0.3726134961398295	No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATCT	83704	0.3673644296924416	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGCA	82619	0.36260252576650853	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGGA	81761	0.3588368911412085	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGCC	81492	0.35765629007570066	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACTC	78503	0.34453801280877544	Illumina PCR Primer Index 6 (96% over 29bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAGGCACCATCAATATC	77629	0.3407021565587612	No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGTG	76031	0.3336887717904285	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGTC	75969	0.3334166629946609	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATGG	74889	0.32867670332645105	Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAC	71285	0.312859282359573	Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATTC	70508	0.30944914470938867	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATCG	68724	0.30161943355375315	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACGC	68124	0.2989861226269699	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATTA	67305	0.2953916532119108	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATTG	65914	0.2892867607133183	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGGG	62454	0.2741013343688682	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACTA	60976	0.2676146117858922	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATTT	59993	0.2633003707175123	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGGT	58930	0.2586350215255613	Illumina PCR Primer Index 6 (96% over 29bp)
CCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATATCTCGTATGCCGT	58510	0.256791703876813	No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGGC	58122	0.2550888294774932	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACGT	57807	0.253706341240932	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACGA	57051	0.2503883694731851	Illumina PCR Primer Index 6 (96% over 29bp)
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAT	55178	0.24216805053007673	No Hit
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	54140	0.2376124226267417	Illumina PCR Primer Index 6 (96% over 29bp)
CGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATATCTC	53906	0.23658543136529622	No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACCG	53773	0.23600171410985926	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGTT	50232	0.22046079079029346	Illumina PCR Primer Index 6 (96% over 29bp)
CCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATATCTCGTATGCCGTC	49404	0.2168268217113326	No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGCTGTAGGCACCATCA	48349	0.21219658333173874	No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACCT	47369	0.20789550881799274	Illumina PCR Primer Index 6 (96% over 29bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCACCATCAATA	46960	0.20610046853623548	No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGCG	46768	0.20525780903966487	Illumina PCR Primer Index 6 (96% over 29bp)
CGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAGGCACCATCAATATCTCG	45405	0.19927580438432224	No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCTGTAGGCACCATCAATATCTCGTA	44222	0.19408379300701462	No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATCAA	44086	0.19348690919694372	No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	44038	0.1932762443228011	Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACTG	43803	0.19224486420981096	Illumina PCR Primer Index 6 (96% over 29bp)
TTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATATCTCGTATG	42742	0.18758829272094926	No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGCT	39706	0.17426373943142603	Illumina PCR Primer Index 6 (96% over 29bp)
CCGTCCCCGCTCGGCGGGGTCCCCCTGTAGGCACCATCAATATCTCGTAT	39543	0.17354835662964993	No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACGG	37176	0.16315994502349002	Illumina PCR Primer Index 6 (96% over 29bp)
TGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATATCTCGTATGC	36262	0.1591485347116902	No Hit
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAT	36186	0.15881498199429764	Illumina PCR Primer Index 6 (100% over 28bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAG	35985	0.15793282283382526	Illumina PCR Primer Index 6 (100% over 28bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATAAA	35195	0.15446563011356065	Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACTT	35142	0.1542330209816948	Illumina PCR Primer Index 6 (96% over 29bp)
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA	34089	0.14961156030519018	Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATAAC	33857	0.148593346746834	Illumina PCR Primer Index 6 (100% over 28bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCACCATCAATATC	33837	0.14850556971594123	No Hit
GAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA	33381	0.146504253411586	Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAC	33135	0.14542459593160484	Illumina PCR Primer Index 6 (100% over 28bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACA	32169	0.14118496533948383	Illumina PCR Primer Index 6 (100% over 28bp)
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAC	32106	0.14090846769217155	Illumina PCR Primer Index 6 (96% over 29bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAGGCACCATCAATAT	31629	0.13881498550537888	No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGCTGTAGGCACCATC	31290	0.13732716483174637	No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCTGTAGGCACCATCAATATCT	30161	0.13237215143784922	No Hit
CCGGATAGCCGGGTCCCCTGTAGGCACCATCAATATCTCGTATGCCGTCT	29695	0.13032694661804756	Illumina PCR Primer Index 6 (95% over 21bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACC	28409	0.12468288353164213	Illumina PCR Primer Index 6 (100% over 28bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCTAGATCGGAAGAGCGTCG	27000	0.11849899170524614	Illumina Single End PCR Primer 1 (100% over 33bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAGGCACCATCAATATCT	25417	0.11155143971008301	No Hit
TTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATATCTCGTAT	24822	0.10894007304102293	No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA	24617	0.108040358474372	No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATATC	24024	0.10543776950840122	No Hit
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA	23622	0.10367345118745645	Illumina PCR Primer Index 6 (96% over 29bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCTGTAGGCACCATCAATATCTC	23262	0.10209346463138651	No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAAA	23230	0.10195302138195805	Illumina PCR Primer Index 6 (100% over 28bp)
GTTGGCCCCGGATAGCCGGGTCCCTGTAGGCACCATCAATATCTCGTATG	23003	0.10095675208132507	No Hit

Adapter Content

Adapter graph

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content