SRR14585408_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR14585408_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23276662
Total Bases	3.4 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	57

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	919136	3.9487448844684003	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC	707737	3.0405433562595876	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAG	407071	1.7488375266178628	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAACA	357094	1.5341289055965155	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAACC	332421	1.4281300299845399	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAT	325585	1.3987615578213062	Illumina PCR Primer Index 6 (96% over 29bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA	282357	1.2130476440307463	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAGA	275595	1.1839970868675243	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAATA	269441	1.1575585880827757	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACAC	252878	1.0864014780126119	Illumina PCR Primer Index 6 (96% over 29bp)
CGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATATCTCGT	250327	1.0754420028095093	No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAA	225609	0.9692498005083375	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACAA	224556	0.9647259559811454	Illumina PCR Primer Index 6 (96% over 29bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATATCT	222098	0.9541660226023818	No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAATC	220364	0.9467165008453531	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATAC	220249	0.9462224437507405	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATAA	217667	0.9351297879395251	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAGC	212163	0.9114837857765	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAATG	202744	0.871018361653402	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATAT	196516	0.8442619478686421	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAGG	184259	0.7916040538802342	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAC	172662	0.741781618000038	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAGT	172365	0.7405056618513428	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAACG	164256	0.7056681924581798	Illumina PCR Primer Index 6 (96% over 29bp)
CCGTCCCCGCTCGGCGGGGTCCCCCTGTAGGCACCATCAATATCTCGTAT	163067	0.7005600717147502	No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACCC	158582	0.681291845024858	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATAG	156068	0.6704913273217612	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAG	141856	0.6094344627249388	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGTA	138574	0.5953345028595595	Illumina PCR Primer Index 6 (96% over 29bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATATC	136914	0.5882028961025425	No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAATT	132950	0.5711729628586779	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATCC	127096	0.5460233086685711	Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	123084	0.5287871602895639	Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACCA	120975	0.5197265827892332	Illumina PCR Primer Index 6 (96% over 29bp)
CGTCCCCGCTCGGCGGGGTCCCCCTGTAGGCACCATCAATATCTCGTATG	118046	0.5071431633968823	No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAACT	117126	0.5031907066399813	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATGC	116366	0.4999256336668892	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATGA	115464	0.4960505075856667	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATCA	115263	0.4951869817072568	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGGA	113044	0.48565382785555766	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATGT	110652	0.47537744028761514	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACAG	109385	0.4699342199495787	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAT	107594	0.46223981771956824	Illumina PCR Primer Index 6 (96% over 29bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATAT	104992	0.45106123893537653	No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGCA	104755	0.45004305170560965	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGCC	101368	0.43549199623210577	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGTG	100907	0.43351147170500653	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATGG	100341	0.4310798515697827	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACAT	95060	0.40839189055544134	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGTC	93362	0.4010970301497697	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGGG	92563	0.39766440737937425	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATCT	89618	0.38501224960864233	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACGC	86910	0.37337827906767734	Illumina PCR Primer Index 6 (96% over 29bp)
GTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATATCTCGTAT	86844	0.37309473325685616	No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACTC	86025	0.36957618751348453	Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAC	85018	0.3652499658241375	Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATCG	82995	0.35655885710760415	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGGC	81884	0.3517858359587814	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATTG	79331	0.3408177684583812	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGGT	78663	0.33794794116097915	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATTC	76191	0.32732786170113226	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACGA	73673	0.31651015940344024	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATTA	73236	0.31463274244391226	Illumina PCR Primer Index 6 (96% over 29bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATATCTC	69807	0.29990124872715856	No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGCTGTAGGCACCATCA	69749	0.29965207210552786	No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACGT	68695	0.29512393142968696	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACCG	66533	0.2858356580509697	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACTA	66468	0.28555640838879737	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATTT	65927	0.2832321919697936	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGCG	65434	0.2811141906859326	Illumina PCR Primer Index 6 (96% over 29bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCACCATCAATATC	61767	0.26536021359076317	No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGTT	60801	0.26121013399601717	Illumina PCR Primer Index 6 (96% over 29bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATA	56194	0.24141777717097065	No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCACCATCAAT	54989	0.23624091804916014	No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACTG	53582	0.23019623690029092	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACGG	51829	0.22266508831893508	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACCT	49688	0.2134670340618427	Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAG	49329	0.21192471669692156	Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGCT	48927	0.2101976649401018	Illumina PCR Primer Index 6 (96% over 29bp)
TCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATATCTCG	48721	0.2093126583184479	No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCTGTAGGCACCATCAATATCTCGTAT	46938	0.20165262527762784	No Hit
TGCCACATCGAGAACTGGGAGAACTGTCATGAGAACATCTCGTATGCCGT	46652	0.20042392676406953	No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAGGCACCATCAATATC	44403	0.19076188845290618	No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCTGTAGGCACCATCAATATCTCGTA	44149	0.1896706666961096	No Hit
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAT	44067	0.18931838250690758	Illumina PCR Primer Index 6 (100% over 28bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	43933	0.18874269858796766	Illumina PCR Primer Index 6 (100% over 28bp)
GAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA	43751	0.18796079953388506	Illumina PCR Primer Index 6 (96% over 29bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAAT	43443	0.186637585750053	No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAGGCACCATCAATATCTCG	41249	0.17721183561457396	No Hit
AGCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAA	38887	0.1670643325060956	Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACA	38678	0.16616643743849527	Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACTT	38127	0.16379925953300348	Illumina PCR Primer Index 6 (96% over 29bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATAAA	37742	0.1621452423032134	Illumina PCR Primer Index 6 (100% over 28bp)
CGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATATCTC	36193	0.15549050804621384	No Hit
CCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATATCTCGTATGCCGT	35335	0.1518044125055388	No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATAAC	35162	0.15106117878929548	Illumina PCR Primer Index 6 (100% over 28bp)
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCACCATCAATAT	34580	0.14856082027569073	No Hit
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACC	33635	0.14450095980256963	Illumina PCR Primer Index 6 (100% over 28bp)
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA	33615	0.1444150368295935	Illumina PCR Primer Index 6 (96% over 29bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA	33465	0.14377061453227272	No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGCTGTAGGCACCATC	32248	0.13854220162667655	No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAC	31774	0.13650582716714277	Illumina PCR Primer Index 6 (100% over 28bp)
AGCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATAT	30578	0.13136763338317153	No Hit
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGA	29416	0.12637550865325964	Illumina PCR Primer Index 6 (100% over 28bp)
TCGTCCCCGCTCGGCGGGGTCCCCCTGTAGGCACCATCAATATCTCGTAT	28796	0.1237118964910003	No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAAA	27742	0.1191837558151594	Illumina PCR Primer Index 6 (100% over 28bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATA	26698	0.11469857662580658	Illumina PCR Primer Index 6 (100% over 28bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCACCATCAATA	26298	0.11298011716628441	No Hit
GCCGGGTTTCGCCCCCGCGGCGTCCTGTAGGCACCATCAATATCTCGTAT	26149	0.11233999101761241	No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAA	25456	0.10936276000399026	No Hit
CCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATATCTCGTATGCCGTC	25366	0.10897610662559777	No Hit
GTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAA	24095	0.10351570169296612	Illumina PCR Primer Index 6 (96% over 29bp)

Adapter Content

Adapter graph

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content