SRR14585414_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR14585414_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	37734102
Total Bases	5.6 Gbp
Sequences flagged as poor quality	0
Sequence length	150
%GC	56

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	1493616	3.9582656558250675	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC	1224204	3.2442908009312106	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAACA	624964	1.6562312785395026	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAACC	618003	1.6377837744754071	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAG	599133	1.5877759592635858	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAT	573548	1.519972570170081	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAATA	490685	1.30037545348237	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACAC	469259	1.2435939246679304	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATAC	446793	1.1840562682530513	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAATC	427323	1.1324583794255922	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATAA	414570	1.0986613647252028	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAGA	399515	1.058763767586148	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACAA	393805	1.043631567010658	Illumina PCR Primer Index 6 (96% over 29bp)
GCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAA	384009	1.0176709651126719	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATAT	382149	1.0127417369041933	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAATG	337087	0.8933219081243804	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAA	326840	0.8661661008919731	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAGC	326784	0.8660176940211801	Illumina PCR Primer Index 6 (96% over 29bp)
CCGTCCCCGCTCGGCGGGGTCCCCCTGTAGGCACCATCAATATCTCGTAT	308308	0.8170540271502948	No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACCC	308062	0.8164020969678834	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAGT	280476	0.7432958123662251	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATCC	275700	0.7306388263857452	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATAG	267167	0.7080253294486775	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAC	258918	0.6861644673563452	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAACG	256975	0.6810152789643702	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAGG	254241	0.6737698435224456	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAATT	247581	0.6561200263888617	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATCA	234987	0.6227443811966162	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGTA	229039	0.6069814514202564	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAACT	226890	0.601286337753579	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACCA	224634	0.5953076609587794	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATGC	207169	0.5490232681302446	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATGT	197003	0.5220821208359484	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATGA	194376	0.5151202485221458	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAG	193221	0.5120593568120424	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATCT	187572	0.4970888137208088	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACAT	182364	0.4832869747370694	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACTC	178548	0.4731741065416106	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACAG	172198	0.4563458274427731	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAT	171097	0.45342804235807704	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATTC	169926	0.4503247486848899	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGCC	166047	0.440044922759789	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGCA	161664	0.4284294349975521	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGTC	161106	0.42695066653500857	Illumina PCR Primer Index 6 (96% over 29bp)
TCGTCCCCGCTCGGCGGGGTCCCCCTGTAGGCACCATCAATATCTCGTAT	158831	0.42092163740904714	No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATGG	157524	0.41745792704964857	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGTG	154975	0.41070276430587904	Illumina PCR Primer Index 6 (96% over 29bp)
CGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATATCTCGT	154958	0.4106577122201026	No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATTA	153490	0.40676733210717453	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGGA	152707	0.4046922860387667	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATCG	150095	0.39777016556535516	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATTG	148403	0.39328615796925553	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACGC	142589	0.3778783446337215	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATTT	138316	0.3665543703676849	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACTA	132407	0.35089479537634155	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGGG	121537	0.3220879617063631	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACGT	120921	0.32045548612764124	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGGT	118837	0.3149326304359913	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGGC	115824	0.30694781076279487	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACGA	113873	0.30177742138927804	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACCG	111704	0.29602930526874605	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGTT	107190	0.2840666514337614	Illumina PCR Primer Index 6 (96% over 29bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATATCT	105921	0.28070364573668666	No Hit
TGCCACATCGAGAACTGGGAGAACTGTCATGAGAACATCTCGTATGCCGT	102770	0.2723531091318935	No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACCT	101963	0.27021446011886013	Illumina PCR Primer Index 6 (96% over 29bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	97801	0.2591846494717166	Illumina PCR Primer Index 6 (100% over 28bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACTG	94800	0.2512316312708329	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGCG	94616	0.25074400869537056	Illumina PCR Primer Index 6 (96% over 29bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCTGTAGGCACCATCAATATCTCGTA	89999	0.23850839222303472	No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAGCT	84484	0.2238929655726271	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACTT	78778	0.208771365487908	Illumina PCR Primer Index 6 (96% over 29bp)
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAACGG	75835	0.20097205440320268	Illumina PCR Primer Index 6 (96% over 29bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATATC	73819	0.19562940705465842	No Hit
CGTCCCCGCTCGGCGGGGTCCCCCTGTAGGCACCATCAATATCTCGTATG	73484	0.19474161595259376	No Hit
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAC	71846	0.1904007149819015	Illumina PCR Primer Index 6 (100% over 28bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	69575	0.18438228634671097	Illumina PCR Primer Index 6 (100% over 28bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATAAC	65083	0.17247793521096647	Illumina PCR Primer Index 6 (100% over 28bp)
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATAAA	64329	0.17047974270064783	Illumina PCR Primer Index 6 (100% over 28bp)
AGCGGGCCCCCGGTGGCTGTAGGCACCATCAATATCTCGTATGCCGTCTT	57264	0.1517566258765082	TruSeq Adapter, Index 6 (95% over 22bp)
TCGTCCCCGCTCGGCGGGGTCCCCGCTGTAGGCACCATCAATATCTCGTA	55330	0.14663128858876778	No Hit
GCGTCCCCGCTCGGCGGGGTCCCCCTGTAGGCACCATCAATATCTCGTAT	53162	0.1408858225909285	No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAC	51780	0.1372233530295752	Illumina PCR Primer Index 6 (100% over 28bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATAT	50072	0.13269694347039185	No Hit
GTTGGCCCCGGATAGCCGGGTCCCCTGTAGGCACCATCAATATCTCGTAT	49822	0.1320344127972093	No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATTAC	47792	0.12665466373096676	Illumina PCR Primer Index 6 (100% over 28bp)
CGACGCTCTTCCGATCTCTGTAGGCACCATCAATATCTCGTATGCCGTCT	47210	0.1251122923237977	Illumina PCR Primer Index 6 (95% over 21bp)
TCTTCCGATCTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCT	45489	0.12055143116960887	Illumina PCR Primer Index 6 (96% over 27bp)
CACGACGCTCTTCCGATCTCTGTAGGCACCATCAATATCTCGTATGCCGT	45447	0.12044012601651419	No Hit
GAGCGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAA	44729	0.11853733792313384	Illumina PCR Primer Index 6 (96% over 29bp)
CGTCGTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAA	44477	0.11786950700456579	Illumina PCR Primer Index 6 (96% over 29bp)
CTCTTCCGATCTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGC	43765	0.11598261964734181	Illumina PCR Primer Index 6 (96% over 26bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGCTGTAGGCACCATCA	42646	0.1130171323541766	No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCTGTAGGCACCATCAAT	41494	0.10996419101215128	No Hit
CTGTAGGCACCATCAATATTGTAGGCACCATCAATATCTCGTATGCCGTC	40979	0.1085993778253952	No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATCAC	40814	0.10816210758109468	Illumina PCR Primer Index 6 (100% over 28bp)
CGGTTGGCCCCGGATAGCCGGGTCCCCCTGTAGGCACCATCAATATCTCG	40571	0.10751812776676122	No Hit
TGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAATACC	40195	0.10652168163429462	Illumina PCR Primer Index 6 (100% over 28bp)
GACGCTCTTCCGATCTCTGTAGGCACCATCAATATCTCGTATGCCGTCTT	39912	0.10577169691225194	Illumina PCR Primer Index 6 (95% over 22bp)
ACGCTCTTCCGATCTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTC	39741	0.10531852593179507	Illumina PCR Primer Index 6 (95% over 23bp)
GCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAT	37846	0.10029654342907114	Illumina PCR Primer Index 6 (100% over 28bp)
CGCTCTTCCGATCTCTGTAGGCACCATCAATATCTCGTATGCCGTCTTCT	37833	0.10026209183406565	Illumina PCR Primer Index 6 (95% over 24bp)

Adapter Content

Adapter graph

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content