FastQCFastQC Report
Thu 14 Sept 2023
SRR14602133.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14602133.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56839026
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT44151007.767726350553579No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA39698626.984394841670932No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA39425486.936339831016809Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA16066192.8266124757310234No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA11865972.0876448516200825Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC6909051.2155468673935406No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT5985171.0530036176200486No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA5649080.9938734699641053No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC5123040.9013243823002879No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT3655960.64321299242531No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT3223750.5671719286674617No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA3213690.5654020179726514No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATAG2922200.5141185916873382No Hit
CCTACACGACGCTCTTCCGATCTCTGTAGGCACCATCAATAGATCGGAAG2616750.4603791064259264Illumina Single End PCR Primer 1 (100% over 23bp)
CTACACGACGCTCTTCCGATCTCTGTAGGCACCATCAATAGATCGGAAGA2560980.450567186003504Illumina Single End PCR Primer 1 (100% over 22bp)
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGTAGGCACCATCAATAGA2495110.43897831746800164No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2256410.396982523944024No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT2240810.3942379308188708No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2176200.38287074095886164Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCTACACGACGCTCTTCCGATCTCTGTAGGCACCATCAATAGATCGGAA2099990.3694626998006616Illumina Single End PCR Primer 1 (100% over 24bp)
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCACCAT2093880.3683877341599766No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2085020.36682894601325505No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA2074260.3649358804987264No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2022570.3558417767398055No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2010210.3536672144944919Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT2002860.3523740888874486No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATAGAT1892780.3330071138094449No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG1820040.32020956868613476No Hit
TGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAA1663150.29260705487810434No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATCAATAGATC1661860.29238009813890903No Hit
ACCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1656210.2913860628083247No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1643240.2891041799343993No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1531070.26936949975180785No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1529920.2691671739765562No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAG1522940.2679391444885069No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT1404270.24706088383710162No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGAT1339170.23560748560328953No Hit
TACACGACGCTCTTCCGATCTCTGTAGGCACCATCAATAGATCGGAAGAG1338840.23554942690256517Illumina Single End PCR Primer 1 (100% over 21bp)
TCGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGGCACCATC1332400.23441640256115578No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGTAGGCACCATCAATAGATCG1315740.2314853178518576No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGAT1309160.2303276625465046No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCG1308740.2302537696546735No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA1279320.2250777485173655No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATC1203760.2117840654060469No Hit
ACACGACGCTCTTCCGATCTCTGTAGGCACCATCAATAGATCGGAAGAGC1125320.19798368817931536No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG1123540.19767052306631713No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGATCGG1111800.19560504080418267No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTAGGCACCATCAATAGA1109500.19520038925367933No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC1087150.19126823179552727No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAAT1059020.1863191673974146No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGG987060.17365885193036207No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA938940.1651928377520051No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGA935920.16466151267264853No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGTAGGCACCATCAATAG934980.1644961333433124No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAA933990.1643219572411392No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATA927670.1632100451545387No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGTAGGCACCATCAATAGATC915580.16108298548254504No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT907460.1596543895738115No Hit
ACGACGCTCTTCCGATCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC878820.15461559809276113No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG863180.15186396754933837No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT833690.14667563093005853No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA802740.14123042854393741No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA794730.1398211855354453No Hit
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCA768440.13519584237773533No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCA759160.1335631613391827No Hit
CACGACGCTCTTCCGATCTCTGTAGGCACCATCAATAGATCGGAAGAGCA757180.13321480913483633No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGATC724740.12750746291817175No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATAGA697770.12276248364987816No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT689060.12123008582166767No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATAGAT675940.11892181262923118No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA670790.11801574502701717No Hit
CTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA668090.1175407192938176Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAAT640810.11274120003393444No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC608870.10712182154564012No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCCTGTAGGCACCATCAATAGA607530.10688606803360774No Hit
TGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCACCATCAATAG603060.10609963654197735No Hit
GGGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATC602620.10602222494101148No Hit
TAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT600900.10571961595541768TruSeq Adapter, Index 1 (100% over 35bp)
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATC584480.10283075575573727No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATAG583990.10274454738193439No Hit
TTCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT571010.1004609051534416No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)