FastQCFastQC Report
Thu 14 Sept 2023
SRR14602134.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14602134.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72222936
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT66443809.199819846703546No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA43816486.066837271749794No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA23349943.2330366630345795No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA13928371.9285244787057672Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC12055981.6692730409076695No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT9681661.3405242899568637No Hit
TCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATC9189301.2723520406315245No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA6715040.9297655802860189No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT6135280.8494919120983948No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA4752480.6580291889546003No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT3665080.5074676000432882No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT3575100.49500895394227673No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATAG3269400.4526816799582892No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA3033210.41997877239440945No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT3011320.41694787927203625No Hit
TCGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGGCACCATC2751480.3809703886864971No Hit
CCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA2647390.3665580695860938No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGTAGGCACCATCAATAGA2632300.364468705620054No Hit
ACCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2556680.35399834756094656No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCCTGTAGGCACCATCAATAGATC2549250.3529695884974823No Hit
TGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAA2531850.35056038153862923No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCG2496690.34569212195970545No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGAT2494820.34543320144171374No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATAGATCG2463350.34107585989027084No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA2452860.3396234127064566No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG2392860.33131580250351494No Hit
TCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGCTGTAGGCACCAT2385800.3303382737029688No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGAT2343170.3244357166537788No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT2318260.3209866738178575No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG2295340.31781316672033383No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATAGAT2292810.3174628624901098No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG2099050.29063481994141027Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2092110.28967390636127005No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT2053510.2843293437973776No Hit
TCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGATC1912610.2648203058374697No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC1838020.25449256175351276No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGTAGGCACCATCAATAGATCG1813700.2511252104179204No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAATAGATCGG1811490.25081921344211205No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAAT1720700.2382484146033609No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1705430.23613412780671225No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAG1631080.22583961416356707No Hit
CCTACACGACGCTCTTCCGATCTCTGTAGGCACCATCAATAGATCGGAAG1500590.20777194657386955Illumina Single End PCR Primer 1 (100% over 23bp)
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT1477000.20450567116241303No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTAGGCACCATCAATAGA1434800.19866265198634406No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGTAGGCACCATCA1418660.19642790484175277No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGTAGGCACCATCAATAGATC1417740.19630052148530766No Hit
GGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAA1400470.19390931434856096No Hit
CTACACGACGCTCTTCCGATCTCTGTAGGCACCATCAATAGATCGGAAGA1377520.1907316534459358Illumina Single End PCR Primer 1 (100% over 22bp)
TAGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCA1337610.1852057080592791No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA1322090.18305680622011822No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATAGATCGG1280640.17731763217158605No Hit
CCCTACACGACGCTCTTCCGATCTCTGTAGGCACCATCAATAGATCGGAA1248910.1729242909759304Illumina Single End PCR Primer 1 (100% over 24bp)
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGA1224150.16949601716551652No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATAGATC1182810.16377207373568972No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1164420.16122579120848812No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGCTGTAGGCACCATCAATAGA1136510.15736136786241978No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA1130740.1565624526812369No Hit
TCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGCTGTAGGCACCATCAATA1125610.15585215200888536No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCCTGTAGGCACCATCAATA1117990.15479708551311178No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATAGA1102620.15266895269945824No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCG1074850.14882391377719675No Hit
TCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCAATAGATCG1061080.14691731723562165No Hit
TTCTCGCTTCTGGCGCCAAGCGTCCGTCCCGCTGTAGGCACCATCAATAG1027080.14220967145395474No Hit
TCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGAT1017380.14086660780447918No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATAG989120.13695372339889367No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT974290.1349003590770666No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA966170.1337760624962685No Hit
TCCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGTAGGCACCATC930470.12883303442551824No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGAT916830.1269444377060495No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG892850.12362416282827382No Hit
TCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGTAGGCACCATCAATA876750.1213949540904845No Hit
TGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA873980.12101141941944868No Hit
TGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAAT812340.11247673453762667No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC801000.1109065962092707No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGCTGCCTGTAGGCACCATCAATAG793380.10985152971349711No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATAG793050.10980583785738093No Hit
TGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCACCATCAATAG788910.10923261275337795No Hit
TGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCCTGTAGGCACCATCA772560.10696878897307636No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAA761850.10548588055185129No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)