FastQCFastQC Report
Thu 14 Sept 2023
SRR14624501.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14624501.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34525760
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length64-75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA399390311.567893074620224No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA14657304.245322912515177No Hit
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC14140214.095553580862521No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTCTACATCTCGTA11481773.3255661859434813TruSeq Adapter, Index 1 (97% over 36bp)
TCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCT9875672.860377295097921Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA9067402.6262709350931015Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA8346542.417481903367225Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC7451572.1582638586377243No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA5535371.603257973177129No Hit
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC4197791.215842895275875Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC4128621.1958085788698063No Hit
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA2624930.760281598435487No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC2608030.7553867025664316No Hit
TTTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGT2594430.7514476147664816No Hit
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACA2569880.7443369820099544No Hit
TCCCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC2553450.7395782163810442No Hit
CGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTCTACATCTCGTA2149820.6226713039770885TruSeq Adapter, Index 1 (97% over 36bp)
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACAGATCGGAAGAGCA2143450.620826304764906No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC1634130.4733074666567803No Hit
TGCAATTATTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTC1627620.47142191801136313TruSeq Adapter, Index 1 (97% over 36bp)
TCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACGT1530160.4431937197037806No Hit
TCTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC1490280.43164292400804505No Hit
AATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGA1406630.4074146376502646Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT1154300.33433007702075207Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAACT1152500.3338087271648763Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG1134840.3286937058011178Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACTGAGATCGGAAGAG1130860.3275409433420148No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA1108090.3209458676651868Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA1046880.30321707617732385Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA1044980.3026667624405661Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCAATTATTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCT979970.2838373434791877TruSeq Adapter, Index 1 (97% over 36bp)
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC965500.27964626991556446No Hit
TCGACTCTTTACTTATTCGGTTAAAAGAGAAGATCGGAAGAGCACACGTC935230.27087890317258767No Hit
CATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC865950.2508127264975485No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCT796860.2308015811961851Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAACAGATCGGAAGAGCA790430.2289392036554735No Hit
CTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC731700.21192871641348374No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACACG730340.21153480763348873No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCAC708460.20519751049651044No Hit
GATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC648080.18770911921996794No Hit
AAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC619100.1793153865403687No Hit
AGCAGATATCACCTTATCCGGGATCCAGATCGGAAGAGCACACGTCTGAA614160.17788457082479864Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC576590.1670028407774369No Hit
AAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG559910.16217166544632183Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA546850.1583889826031346No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAAGATCGGAAGAGCACACGTC493850.1430381257356826No Hit
AAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT483320.13998822907880956No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTC479150.1387804352460308No Hit
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC471690.1366197297322347No Hit
TCCCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACACG468080.13557413363239507No Hit
AACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGA463160.13414911069300142Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT443240.12837950562131No Hit
TTCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACG428880.12422029232665698No Hit
CCGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA397650.11517487232721307Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAACAGATCGGAAGAGCA385210.11157176554549414No Hit
TAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG374600.10849869778391553Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC361550.10471891132881651No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)