FastQCFastQC Report
Thu 14 Sept 2023
SRR14624502.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14624502.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31030399
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length65-75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA29383369.469217588855368No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA17003915.479758735941488Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA11267833.631223046793565No Hit
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC9782193.152453824393299No Hit
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC7829812.5232708093763154Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCT7408052.3873524797409145Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA6580162.120552816610576Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAGAGGATCTCGTA5997281.932711210062107TruSeq Adapter, Index 7 (97% over 37bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC5678651.830028031544164No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA4155761.3392544517394056No Hit
TTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC3260431.0507212620759403No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA2323480.7487754185822748Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACAGATCGGAAGAGCA2168110.6987051632819804No Hit
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA2076400.6691502742198062No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC2025890.652872687843943No Hit
TGCAATTATTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAG1940800.6254511906211713TruSeq Adapter, Index 7 (100% over 36bp)
TCCCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC1875240.6043235215892647No Hit
TTTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGT1863680.6005981424860183No Hit
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACA1840530.5931377163406761No Hit
TTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAACT1644450.5299480680219419Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC1376170.44349091353933284No Hit
ACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA1359150.43800596956552185Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACTGAGATCGGAAGAG1300440.4190858132375288No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACGT1235490.39815472562889054No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC1211050.3902785781130304No Hit
AATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGA1172400.3778230502289062Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCAATTATTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA1037530.33435921980893635TruSeq Adapter, Index 7 (100% over 37bp)
CGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAGAGGATCTCGTA999870.3222227339068376TruSeq Adapter, Index 7 (97% over 37bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT947450.3053296220909051Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA891690.28736014641642216Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG808460.26053806140230423Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTCTTTACTTATTCGGTTAAAAGAGAAGATCGGAAGAGCACACGTC758960.2445859623010326No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC729250.23501148019398654No Hit
AGCAGATATCACCTTATCCGGGATCCAGATCGGAAGAGCACACGTCTGAA630970.20333931252382542Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC611480.19705837491809242No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCT601010.19368426425970223Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGA584950.18850869432906744Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA541470.1744966282902131Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACACG538630.1735813967458169No Hit
CTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC538350.1734911626498905No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAACAGATCGGAAGAGCA528740.17039419957184565No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCAC497750.1604072187405647No Hit
AAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG496230.15991737650553575Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC494450.15934374546714658No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAAC450340.14512865271245787Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC428310.13802916295082124No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTC422640.13620192250831192No Hit
ACACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA396400.1277456986614964No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAGAGGATCTGGTA392460.12647597602596086TruSeq Adapter, Index 7 (97% over 37bp)
AAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT385090.1241008857153271No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC383650.12363682465056283No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAAGATCGGAAGAGCACACGTC381690.12300518597907814No Hit
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC379940.12244122287953822No Hit
TTCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACG351760.1133598056538042No Hit
TCCCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACACG349840.11274105756745184No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT340520.10973755123161646No Hit
TGCAATTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGA323700.10431706018346719TruSeq Adapter, Index 7 (100% over 37bp)
ATACCCCAACGCAAGGCGGGGTGCAGATCGGAAGAGCACACGTCTGAACT316420.10197097368938117Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)