FastQCFastQC Report
Thu 14 Sept 2023
SRR14624503.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14624503.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27038334
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length64-75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA313261611.585832174423173No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTACGCTATCTCGTA11527784.263494932786909TruSeq Adapter, Index 6 (97% over 37bp)
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA10784733.9886814032255096No Hit
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC9427063.486553572420549No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCT7216922.6691437423622326Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA6503012.4051075040348273Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA6128342.266537575872833Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC5640642.086164036585982No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA3604431.333081394733862No Hit
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC3197501.1825802580883866Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC3162491.1696319751061584No Hit
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACAGATCGGAAGAGCA3009751.113141808219397No Hit
TGCAATTATTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCT2547490.942177132659135TruSeq Adapter, Index 8 (97% over 36bp)
CGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTACGCTATCTCGTA1903080.7038451407546041TruSeq Adapter, Index 6 (97% over 37bp)
GCAATTATTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTA1850270.6843136119259419TruSeq Adapter, Index 6 (97% over 37bp)
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1835300.6787770282000363No Hit
TTTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGT1801570.6663021471663158No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC1777830.6575220204025884No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACGT1657390.6129778558101989No Hit
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACA1645280.6084990295629901No Hit
TCCCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC1607110.5943820355203837No Hit
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACTGAGATCGGAAGAG1395690.5161893480567257No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT1252340.46317202827659426Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGA1102080.4075990776650662Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC1089780.40304998081612575No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA1055490.39036798642993314Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC1024260.3788177185768916No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG960760.35533254378764606Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAACT944080.3491635246461561Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA818100.30257041724538203Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAACAGATCGGAAGAGCA696370.2575491522517623No Hit
TCGACTCTTTACTTATTCGGTTAAAAGAGAAGATCGGAAGAGCACACGTC680170.2515576588409626No Hit
CTGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA654340.2420045554581876Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCAC647100.23932687568694136No Hit
AAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT644230.23826541975552193No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCT611650.22621586078491376Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC599380.22167785929414144No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC588000.21746902009569083No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT571360.21131479476509166No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTC562440.20801577493642914No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACACG548230.20276027361744997No Hit
TGCAATTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTA531940.19673549413214583TruSeq Adapter, Index 6 (97% over 37bp)
AAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC529590.19586635774230765No Hit
TTCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACG524590.19401713138094973No Hit
CTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC490370.18136102616381616No Hit
AAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG478690.17704123338368405Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC471380.1743376644433788No Hit
GATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC462270.17096837401298468No Hit
AGCAGATATCACCTTATCCGGGATCCAGATCGGAAGAGCACACGTCTGAA439960.16271712598860566Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA430260.15912962684757131No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAAGATCGGAAGAGCACACGTC385470.1425642571025271No Hit
AACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGA360480.13332182374846024Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGCAATTATTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC341150.12617271463545054TruSeq Adapter, Index 6 (100% over 35bp)
TAGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACAGATCGGAAGAGC329910.12201565377511796No Hit
TAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG310790.1149442121692853Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC310390.11479627406037665No Hit
TCCCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACACG290000.10725512895875908No Hit
CCGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA284610.10526166294121524Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC275010.10171114832740805No Hit
GTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC272270.1006977722813839No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)