FastQCFastQC Report
Thu 14 Sept 2023
SRR14624504.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14624504.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25453883
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length56-75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA21786398.559161680754169No Hit
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC8383313.293528928376075No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA7820493.0724153167514756No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGGAATCTCGTA7710723.029290265850597TruSeq Adapter, Index 3 (97% over 37bp)
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA4822341.8945400196897266Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCT3624091.4237866969059298Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC3552321.3955906059598058No Hit
ATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA3141761.234294979669703Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA2847361.1186348267570807No Hit
TGCAATTATTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTA1949080.7657299281213794TruSeq Adapter, Index 3 (97% over 36bp)
TTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC1898140.7457172644346641No Hit
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC1884810.7404803424294831Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1489910.5853370191102081No Hit
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACAGATCGGAAGAGCA1421120.5583116729184305No Hit
GCAATTATTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAG1387320.5450327559060439TruSeq Adapter, Index 3 (97% over 37bp)
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACA1357250.5332192341734265No Hit
CGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGGAATCTCGTA1267900.4981165349113925TruSeq Adapter, Index 3 (97% over 37bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT1151680.4524574894918783Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC1135790.44621482702658766No Hit
TTTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGT1102200.43301841216131937No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG1029540.4044726692583603Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC961390.37769875818161025No Hit
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACTGAGATCGGAAGAG841620.33064503360842823No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA791910.3111155967834063Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC733810.28829000274732147No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC633680.24895219326654403No Hit
ACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA627950.246701063252314Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACGT622880.244709225700456No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCT614000.24122056347945028Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC546610.2147452316017953No Hit
AATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGA530350.20835720821062942Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC519040.20391387828725388No Hit
TCGACTCTTTACTTATTCGGTTAAAAGAGAAGATCGGAAGAGCACACGTC517230.2032027883525669No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC512250.20124630886375958No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGGAATGTCGTA484640.19039924085452894TruSeq Adapter, Index 3 (97% over 37bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCAC484370.19029316666537677No Hit
AAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT431620.16956941304397447No Hit
GATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC423960.16656004901098978No Hit
TTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAACT422480.16597860530748884Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAACAGATCGGAAGAGCA420890.16535394619359253No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC419260.16471357238500703No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT399370.15689944045079487No Hit
CTGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA379150.1489556622853967Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCAATTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAG376100.14775741681534404TruSeq Adapter, Index 3 (97% over 37bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTC367550.14439840082552435No Hit
AGCAGATATCACCTTATCCGGGATCCAGATCGGAAGAGCACACGTCTGAA350830.1378296584454325Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACACG337810.13271452532409297No Hit
CTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC330370.129791592111899No Hit
ACACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA295670.11615909446900498No Hit
GTGCAATTATTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGT268440.10546131605932187TruSeq Adapter, Index 18 (100% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)