FastQCFastQC Report
Thu 14 Sept 2023
SRR14624505.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14624505.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22480519
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length65-75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA232616610.347474629033252No Hit
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC8880093.950126774208371No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA7593243.377697819165118No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTCAGGATCTCGTA5142252.2874249477959117TruSeq Adapter, Index 15 (97% over 40bp)
TCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCT4625842.0577105003670066Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA4361471.9401109022438496Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC3272251.4555936186348724No Hit
ATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA3038401.3515702195309638Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA2900081.290041390948314No Hit
TTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC2005170.8919589445421612No Hit
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACAGATCGGAAGAGCA1698670.7556186758855523No Hit
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC1657210.7371760411759177Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT1567250.6971591714586305Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG1366130.607695044762979Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1317440.5860362921336469No Hit
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACA1222360.5437418949268921No Hit
TTTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGT1205780.5363666203613894No Hit
TCCCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC1105130.4915945223506628No Hit
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACTGAGATCGGAAGAG1006510.4477254284031432No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC982380.4369916904498512No Hit
CGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTCAGGATCTCGTA945320.4205063059264779TruSeq Adapter, Index 15 (97% over 40bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCT921170.4097636713814303Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACGT913060.40615610342448055No Hit
TCTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC906060.403042296309974No Hit
ACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA849030.37767366491850124Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC731290.3252994292525008No Hit
TGCAATTATTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATC699400.31111381369798446TruSeq Adapter, Index 1 (100% over 36bp)
AAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC651920.2899933048698742No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC592690.26364604838527084No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTC587260.2612306237235893No Hit
AATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGA583280.25946020196419844Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTCTTTACTTATTCGGTTAAAAGAGAAGATCGGAAGAGCACACGTC577670.2569647079767153No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA561900.24994974537731982Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT556330.24747204457334815No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCAC549800.2445673073651013No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAACAGATCGGAAGAGCA513460.22840220014493437No Hit
CATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC503740.22407845655164813No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT490020.21797539460721524No Hit
AACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGA465100.20689024127957187Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCAGATATCACCTTATCCGGGATCCAGATCGGAAGAGCACACGTCTGAA394100.1753073405467196Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA387500.1723714652673277Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC374990.16680664712411666No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACACG369560.16439122246243515No Hit
CCTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT342500.15235413381692833Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC342400.15230965085814965No Hit
TTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAACT333510.14835511582272634Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA323920.14408920007585235Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA315870.1405083218941698No Hit
CCTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG306900.13651820049172353Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCAATGAGTTGAATACAAATGATTCTTCGAAGATCGGAAGAGCACACGTC296080.13170514435187194No Hit
GCAATTATTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCT295340.13137597045690982TruSeq Adapter, Index 15 (97% over 37bp)
CTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC293490.1305530357195045No Hit
TTCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACG245090.10902328367063056No Hit
AAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG238410.10605182202421573Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAACAGATCGGAAGAGCA233980.10408122695032085No Hit
TAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG225010.10009110554787459Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)