FastQCFastQC Report
Thu 14 Sept 2023
SRR14624506.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14624506.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23716860
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length64-75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA17979917.581066802266405No Hit
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC8052433.395234445031931No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA6737972.841004247611193No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCT4065221.7140633287880438Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCGCTAATCTCGTA3460271.4589916203072413TruSeq Adapter, Index 8 (97% over 38bp)
ATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA3007781.2682032950398998Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA2754561.1614353670764175No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC2567741.0826643999247794No Hit
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACAGATCGGAAGAGCA2174920.9170353917002504No Hit
TTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC2136460.900819079760137No Hit
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1656120.698288053308912No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA1625730.6854743840457801Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACGT1624230.6848419225816571No Hit
TGCAATTATTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACT1489120.6278740103032189TruSeq Adapter, Index 8 (100% over 36bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT1406230.5929241897957824Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACTGAGATCGGAAGAG1397760.5893528907283679No Hit
TTTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGT1325590.5589230614845304No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG1174440.495192027949737Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACA1051880.4435157099211278No Hit
TCCCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC955570.402907467514671No Hit
ACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA942140.39724482920588977Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC936180.39473184898844116Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC846110.35675464627273595No Hit
AATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGA845160.35635408734545804Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTC830700.3502571588313124No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCT828220.34921148921062906Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT803570.3388180391502079No Hit
TCTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC781750.3296178330520988No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT759890.3204007613149464No Hit
TCGACTCTTTACTTATTCGGTTAAAAGAGAAGATCGGAAGAGCACACGTC627480.26457127967192956No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAACAGATCGGAAGAGCA617520.2603717355501529No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC615580.2595537520565539No Hit
AAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC611010.2576268527958591No Hit
GATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC599480.25276533234163373No Hit
CATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC595600.25112936535443564No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC588050.24794597598501658No Hit
TTCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACG583240.24591788289006217No Hit
CGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTCGCTAATCTCGTA579220.24422288616621257TruSeq Adapter, Index 8 (97% over 38bp)
GCAATTATTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTC549260.231590522522796TruSeq Adapter, Index 13 (97% over 37bp)
AACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGA548380.23121947846384386Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATACCCCAACGCAAGGCGGGGTGCAGATCGGAAGAGCACACGTCTGAACT496990.2095513487029902Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC488840.20611497474792195No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCAC397050.16741254955335572No Hit
AAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG395430.1667294911721029Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATACCCCAACGCAAGGCGGGGTGCAGATCGGAAGAGCACACGTCTGAAC363970.15346466606456335Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC350240.14767553546295756No Hit
CTGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA346630.14615341153930156Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA338530.14273811963303742Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT314670.13267776594372105Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCAGATATCACCTTATCCGGGATCCAGATCGGAAGAGCACACGTCTGAA298480.12585139854095356Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAACT272410.114859218294496Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG265090.11177280634957579Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTACCCCAACGCAAGGCGGGGTGCAGATCGGAAGAGCACACGTCTGAACT262940.1108662782509995Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACACG261460.11024224960639815No Hit
TAGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACAGATCGGAAGAGC261260.11015792141118176No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAAGATCGGAAGAGCACACGTC250730.10571804193303835No Hit
ACACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA248140.104625991804986No Hit
GTGCAATTATTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAC243730.1027665551004644TruSeq Adapter, Index 5 (100% over 35bp)
AATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACGTCT241500.10182629572380154Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)