FastQCFastQC Report
Thu 14 Sept 2023
SRR14624507.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14624507.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26246922
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length54-75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA21892178.340852310225175No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA10884224.1468557722692205Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA7666012.920727238035759No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCT7125622.7148402391716635Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC6250762.3815211551282087No Hit
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC5180071.9735914176908058Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA4963051.8909074366891476Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC4415621.6823382185537794No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGGCGAATCTCGTA4324841.6477513058483582TruSeq Adapter, Index 3 (97% over 39bp)
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA2918241.1118408474715626No Hit
TTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC2807761.0697482927712438No Hit
TGCAATTATTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAA1986480.7568430309656881TruSeq Adapter, Index 3 (97% over 36bp)
TCCCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC1902040.7246716395926349No Hit
TTTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGT1510820.5756179715091926No Hit
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACA1465390.5583092752742589No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA1434280.54645645687521Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC1419400.5407872206882011No Hit
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACAGATCGGAAGAGCA1314730.5009082588807937No Hit
GCAATTATTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG1303680.49669824141665064TruSeq Adapter, Index 3 (97% over 37bp)
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1243930.4739336673458321No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC1216830.46360864713965316No Hit
TTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAACT1053660.4014413575809003Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGA945110.3600841271978482Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA911700.34735501557096865Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGGCGAATCTCGTA789210.3006866862331514TruSeq Adapter, Index 3 (97% over 39bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG696990.2655511377676971Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA692990.2640271495453829Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT662950.2525819979958031Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACACG592710.2258207648119654No Hit
TCTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC590300.22490256190802108No Hit
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACTGAGATCGGAAGAG550170.20961315006765366No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC535160.20389438426341952No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCT506950.1931464573255485Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCAGATATCACCTTATCCGGGATCCAGATCGGAAGAGCACACGTCTGAA467320.17804754401297035Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC414550.15794232939009No Hit
AACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGA408840.15576683620273646Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCAATTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAG402640.1534046544581494TruSeq Adapter, Index 3 (97% over 37bp)
TCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACGT394190.15018522933851064No Hit
TCGACTCTTTACTTATTCGGTTAAAAGAGAAGATCGGAAGAGCACACGTC389050.14822690447283687No Hit
CATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC372080.14176138443966876No Hit
TCCCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACACG367660.14007737745401155No Hit
CCGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA363990.13867911826003826Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAAC338310.12889511387278096Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG336150.1280721602327313Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCAC321020.12230767478182776No Hit
GTGCAATTATTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA314880.11996835286057542TruSeq Adapter, Index 10 (100% over 35bp)
ACACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA308370.11748806202875903No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAAGATCGGAAGAGCACACGTC307150.11702324562095319No Hit
AAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC279440.10646581721087142No Hit
GATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC275490.10496087884133613No Hit
TAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG265920.10131473701944936Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)