FastQCFastQC Report
Thu 14 Sept 2023
SRR14624508.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14624508.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23195914
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length64-75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA252291310.876540583828687No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA9738134.19820921908919No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCT9486124.089565084609298Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA7574593.2654846021588115Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC5875852.533140103899333No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC5777152.4905895064104824No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACTAGATCTCGTA5465042.3560356362762858TruSeq Adapter, Index 22 (97% over 38bp)
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA4105351.7698591226023686Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA3908971.685197660243093No Hit
TTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC3873271.6698070185981893No Hit
TGCAATTATTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGT2709831.1682359229302195TruSeq Adapter, Index 22 (100% over 36bp)
TTTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGT2175050.9376866977520265No Hit
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACAGATCGGAAGAGCA2123820.9156009114363848No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC2101840.9061251046197187No Hit
TCCCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC1907450.8223215519767836No Hit
GCAATTATTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA1856390.8003090544308795TruSeq Adapter, Index 22 (100% over 37bp)
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1730720.746131409178358No Hit
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC1652330.7123366641211034Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGA1547230.6670269600068357Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACA1474590.6357110998083542No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC1424910.6142935346285557No Hit
CGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACTAGATCTCGTA1173990.5061193104958054TruSeq Adapter, Index 22 (97% over 38bp)
CTGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA1121940.48368001364378227Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA1063000.4582703660653337Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC843620.363693364270966No Hit
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACTGAGATCGGAAGAG839780.36203790029571586No Hit
TCTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC793650.3421507770722033No Hit
CTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC669040.28843010885451636No Hit
TGCAATTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTA666090.28715833314436323TruSeq Adapter, Index 22 (100% over 37bp)
AAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG634960.2737378660741715Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACACG611990.2638352599513863No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA570050.24575448934670133Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT490060.2112699676330926Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGA480790.20727357413034037Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG457890.19740114573627063Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA455940.1965604804363389Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACACG428510.1847351218839663No Hit
GTGCAATTATTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG411430.177371756077385TruSeq Adapter, Index 2 (100% over 35bp)
TCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACGT381960.16466693228816073No Hit
TTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAACT381910.16464537676764968Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC381300.16438239941741464No Hit
CATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC380800.16416684421230393No Hit
AGCAGATATCACCTTATCCGGGATCCAGATCGGAAGAGCACACGTCTGAA376040.16211475865965014Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC361120.15568259133914705No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAAGATCGGAAGAGCACACGTC353540.15241477442966894No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCAC350890.15127233184258226No Hit
GTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC343510.14809073701514844No Hit
ACACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA327480.1411800371392996No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACACG323240.13935212899996094No Hit
TCGACTCTTTACTTATTCGGTTAAAAGAGAAGATCGGAAGAGCACACGTC308860.13315276130097742No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCT293580.12656539423279461Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG293480.1265222831917725Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACGTCT244280.10531165100888028Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC232090.10005641510828157No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)