FastQCFastQC Report
Thu 14 Sept 2023
SRR14624509.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14624509.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23067256
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length55-75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA20605678.932865703662369No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAGAAATCTCGTA11986195.19619238629857TruSeq Adapter, Index 13 (97% over 38bp)
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC7152443.1006895661972105No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA6423682.7847612217075146No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCT5176002.2438733068207157Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA3991021.730166778397916Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC3301241.4311368460990765No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA2633101.1414881770072696Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA2486781.0780562716258926No Hit
CGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAGAAATCTCGTA2170940.9411349143565234TruSeq Adapter, Index 13 (97% over 38bp)
TTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC2078590.9010998100510959No Hit
TGCAATTATTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGG1573290.6820447130772728TruSeq Adapter, Index 15 (97% over 36bp)
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACAGATCGGAAGAGCA1559790.6761922614462682No Hit
TCCCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC1385520.6006436136140336No Hit
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC1364480.5915224593683791Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT1283570.5564467659265584Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGT1251870.5427043424670884No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG1234160.5350267929570817Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACGT1222420.5299373276127859No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAGAAATGTCGTA1190690.516181898705247TruSeq Adapter, Index 13 (97% over 38bp)
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACA1111040.48165243408231995No Hit
GCAATTATTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGC1052480.456265799451829TruSeq Adapter, Index 13 (97% over 37bp)
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1003210.4349065185733405No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC948680.4112669491334383No Hit
ACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA926190.40151719823112036Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC889150.3854598050153863No Hit
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACTGAGATCGGAAGAG885280.3837821022144983No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCT814700.35318461805773516Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGA698490.30280584738817656Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTC654970.2839392773895604No Hit
TCTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC635860.27565480696967165No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAACAGATCGGAAGAGCA599850.2600439341376365No Hit
AAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT589740.2556610981384175No Hit
AAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC581970.25229268708857266No Hit
TCGACTCTTTACTTATTCGGTTAAAAGAGAAGATCGGAAGAGCACACGTC558210.24199237221800457No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT551090.23890574587631924No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC544260.23594483886596654No Hit
CTGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA524970.22758233575766446Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACACG486880.21106975185951898No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCAC475600.2061797033856129No Hit
AACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGA419600.1819028669903347Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAACT419360.18179882340578352Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC411930.17857780743405283No Hit
CGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAGAAATGTCGTA374660.16242070578312393TruSeq Adapter, Index 13 (97% over 38bp)
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC371540.16106813918395843No Hit
CCGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA355900.15428796559070573Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACG332480.14413504579825184No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA327560.14200215231495242Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT313470.13589392687192617Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCAATGAGTTGAATACAAATGATTCTTCGAAGATCGGAAGAGCACACGTC307910.13348358382982353No Hit
CCTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG301910.13088249421604373Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC290890.12610515962540148No Hit
AGCAGATATCACCTTATCCGGGATCCAGATCGGAAGAGCACACGTCTGAA285710.12385955225883824Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA280790.1217266587755388No Hit
AAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG256330.11112288345002978Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGCAATTATTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG251640.10908969840192521TruSeq Adapter, Index 13 (100% over 35bp)
GATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC251120.10886427063539764No Hit
TAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG248280.10763308821820854Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCAATTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGC242820.10526609666966891TruSeq Adapter, Index 13 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)