FastQCFastQC Report
Thu 14 Sept 2023
SRR14624510.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14624510.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28508175
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length67-75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA23523788.251590991005212No Hit
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC9428533.307307465314774No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA7591922.6630676990021285No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCT6936742.433245902271892Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTGAGCATCTCGTA5812622.038930938230876TruSeq Adapter, Index 6 (97% over 36bp)
ATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA5583251.9584733151104903Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC3193531.1202155171279817No Hit
TTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC3178721.115020516044959No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA3174091.1133964205004354No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA2798770.9817429561871288Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA2674220.9380537337097166Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCAATTATTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCC2618870.9186382502562861TruSeq Adapter, Index 6 (97% over 36bp)
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACAGATCGGAAGAGCA2378690.834388732354842No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACGT2240900.7860552280179283No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT2128880.746761236031419Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG1958580.6870239852252906Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGT1924360.6750204108119864No Hit
GCAATTATTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCT1558620.5467273860918841TruSeq Adapter, Index 6 (97% over 36bp)
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACTGAGATCGGAAGAG1489970.5226465741844225No Hit
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1416220.4967768017419565No Hit
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC1373960.4819529836617041Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC1346890.47245746176316095No Hit
AACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGA1331230.4669643005909709Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCT1322320.46383888130334544Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACA1217530.4270810039576367No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTC1173100.41149600070856873No Hit
AATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGA1162280.40770059816175536Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT1132840.3973737357792984No Hit
CCGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA1081030.3792000014031063Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT1065610.37379102660903407No Hit
CGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTGAGCATCTCGTA1007490.35340389204149336TruSeq Adapter, Index 6 (97% over 36bp)
AAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC1000420.35092390165277154No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC949800.3331675914014138No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC921770.32333532399039927No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC896200.31436596695509267No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAACAGATCGGAAGAGCA827640.29031672493942523No Hit
TCTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC808700.2836730165996245No Hit
CTGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA757180.2656010074303248Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACG707240.248083225250301No Hit
TCGACTCTTTACTTATTCGGTTAAAAGAGAAGATCGGAAGAGCACACGTC612280.21477348164166943No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCAC586680.20579360130909816No Hit
CATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC547080.191902848919652No Hit
CTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC542250.19020859806003015No Hit
TAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG528270.18530474153466506Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT506790.17777006069311696Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG493120.17297494490615412Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG467770.1640827587174556Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA433080.15191431931367055Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCAATGAGTTGAATACAAATGATTCTTCGAAGATCGGAAGAGCACACGTC428170.15019200632800941No Hit
GATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC427110.1498201831579889No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACACG415360.14569855839596887No Hit
GTGCAATTATTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC399110.13999843904423906TruSeq Adapter, Index 5 (97% over 35bp)
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC398470.1397739420359248No Hit
ACGATGCCATCTCGCGATTCGGAGGGTAGATCGGAAGAGCACACGTCTGA394540.1383953900942449Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCAGATATCACCTTATCCGGGATCCAGATCGGAAGAGCACACGTCTGAA390340.13692212847718244Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAACT359540.1261182099520576Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGCAATTATTAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCT358650.12580601879987058TruSeq Adapter, Index 6 (97% over 36bp)
TAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG341340.11973407627812022Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACGTCT330550.11594919702857162Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTGAGCATCTGGTA328350.11517748856249127TruSeq Adapter, Index 6 (97% over 36bp)
ACACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA315990.11084189008942172No Hit
ATACCCCAACGCAAGGCGGGGTGCAGATCGGAAGAGCACACGTCTGAACT314360.11027012427137128Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG298550.10472434661285754Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC289580.1015778807307027No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)