FastQCFastQC Report
Thu 14 Sept 2023
SRR14624511.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14624511.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33723006
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length40-75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA25519267.5673147287047895No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA11058393.2791827632447714Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC10435083.0943504858374724No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA9204512.729445293222081No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCT8041382.384538317847466Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA6444921.9111344937636936Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACTCCTATCTCGTA5834171.7300266767440602TruSeq Adapter, Index 4 (97% over 37bp)
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC4679251.3875542411610637Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC4210781.2486372063036135No Hit
TTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC3386421.0041868746813378No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA3358150.9958038734743873No Hit
ACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA2584770.7664708181708357Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGT2115330.6272661458471407No Hit
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACAGATCGGAAGAGCA1935190.5738486064972974No Hit
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1785240.5293834126174873No Hit
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACA1727220.51217854066746No Hit
TGCAATTATTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGA1709510.5069269329074638TruSeq Adapter, Index 2 (97% over 36bp)
TCCCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC1636790.4853630189432105No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC1405430.4167570352417575No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT1370260.4063279530893539Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG1357870.402653903391649Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACGT1305880.38723712826786555No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA1280500.37971110879024245Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGA1231440.3651631767346007Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGA1210010.3588084644648819Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACTGAGATCGGAAGAG1179150.3496574415697106No Hit
TTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAACT1080050.32027097465747867Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC1065190.3158644872879956No Hit
CGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACTCCTATCTCGTA1050400.3114787572614375TruSeq Adapter, Index 4 (97% over 37bp)
TCTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC942280.2794175584465987No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCT941920.27931080639727074Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCAATTATTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAC936440.2776858029797225TruSeq Adapter, Index 4 (97% over 37bp)
CCGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA929810.2757197860712654Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA770370.2284404895577814Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC699990.20757046391415998No Hit
TCGACTCTTTACTTATTCGGTTAAAAGAGAAGATCGGAAGAGCACACGTC698070.2070011196510774No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC677450.20088659949234655No Hit
CATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC648650.1923464355461076No Hit
CTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC640150.1898259010480857No Hit
AGCAGATATCACCTTATCCGGGATCCAGATCGGAAGAGCACACGTCTGAA586800.17400584040461875Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTC558960.16575034858992108No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAACAGATCGGAAGAGCA543700.16122524783229586No Hit
AAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT541990.16071817559798793No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT518370.15371405502819055No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC515750.1529371373358591No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCAC515030.15272363323720312No Hit
GATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC511380.15164128607040547No Hit
AAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG479040.14205139363910796Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACACG478420.14186754288748757No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAAGATCGGAAGAGCACACGTC470220.1394359684305723No Hit
TAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG439420.1303027375436223Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG424210.12579246345951484Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACG355340.10537020335613023No Hit
ACGATGCCATCTCGCGATTCGGAGGGTAGATCGGAAGAGCACACGTCTGA351120.10411883211122995Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA349060.10350797316229757No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)