FastQCFastQC Report
Thu 14 Sept 2023
SRR14624512.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14624512.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24345757
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length40-75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA18713527.686563206886523No Hit
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC7081672.908790225746523No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA7031062.8880022091734507No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA5495632.2573255783338344Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGCATGATCTCGTA5262372.1615142219648376TruSeq Adapter, Index 7 (97% over 36bp)
TCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCT5103872.096410475139467Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA4021921.652000387582937Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC3098041.2725174246995072No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA2767501.1367483869981942No Hit
TTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC2302110.9455898208464004No Hit
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC2075670.8525797739622556Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGT1488040.6112112266626172No Hit
TGCAATTATTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG1470250.6039039985489053TruSeq Adapter, Index 10 (100% over 36bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT1300900.5343436229976337Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1254440.515260215568569No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG1221990.5019314043099995Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC1195570.4910794106751332No Hit
ACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA1153670.47386901955852107Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACA1111160.4564080714351991No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC1084240.4453507032046693No Hit
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACAGATCGGAAGAGCA1072550.44054904515805365No Hit
CGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGCATGATCTCGTA1056290.43387026330707235TruSeq Adapter, Index 7 (97% over 36bp)
TCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACGT1022960.42017999276013474No Hit
GCAATTATTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGG980950.4029244192324765TruSeq Adapter, Index 7 (97% over 36bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCT837480.3439942327527544Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC813660.33421018701533906No Hit
AATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGA787790.3235841054357028Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA772120.3171476656076046Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC696550.28610734921900355No Hit
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACTGAGATCGGAAGAG679340.2790383556362614No Hit
AAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC676320.27779789307845304No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC654340.26876962585307984No Hit
CTGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA615980.2530132868737661Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT568780.23362592504311944No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT553560.2273743223511185No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTC543390.22319700307532028No Hit
AACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGA503020.20661505822143875Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTCTTTACTTATTCGGTTAAAAGAGAAGATCGGAAGAGCACACGTC496360.2038794686071992No Hit
CCGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA493200.20258150116260507Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAACT486750.1999321688785442Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAACAGATCGGAAGAGCA477940.19631346850295106No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC426410.17514756267385728No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCAC409050.16801695671241607No Hit
AGCAGATATCACCTTATCCGGGATCCAGATCGGAAGAGCACACGTCTGAA408920.1679635593175435Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC406440.1669449013230519No Hit
CTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC392220.16110404782237825No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACACG389630.16004020741684064No Hit
AAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG324740.1333866923916147Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT321700.13213801484997983Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACG309110.1269666825311696No Hit
GATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC307000.12610000173746908No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAAGATCGGAAGAGCACACGTC304860.12522099846802873No Hit
CCTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG301280.12375051636307716Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG278580.11442650971994833Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA246600.10129075058130253No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)