FastQCFastQC Report
Thu 14 Sept 2023
SRR14624513.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14624513.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30138160
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length47-75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA17632815.850659097967494No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA11977723.974270492956438Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGGCGAATCTCGTA10813283.587903176570832TruSeq Adapter, Index 3 (97% over 39bp)
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC7606832.5239862022100885No Hit
ACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA6059842.0106867837983473Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATCGTCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA5759421.9110058477358935TruSeq Adapter, Index 10 (100% over 35bp)
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA4915971.631144701600894No Hit
AACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGA3598901.1941339484560438Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCT2903640.9634430237280577Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC2857990.9482961136313565No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT2805310.930816612560289Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC2741310.909581075951551Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG2461380.8166988296564888Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCT1764840.5855831941963279Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG1677060.5564573285164058Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC1476690.48997350866808065No Hit
TTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC1456120.4831482744799285No Hit
ACGATGCCATCTCGCGATTCGGAGGGTAGATCGGAAGAGCACACGTCTGA1425100.4728556753298808Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA1379630.4577684901798915Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACA1343320.4457206412070279No Hit
TAAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCT1269320.4211670520031747Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA1239940.411418613478726Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC1223000.40579783238260064No Hit
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1135250.3766819208604639No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC1102060.3656693042972763No Hit
TTTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGT1077070.35737749086208315No Hit
TCTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC1042120.3457808970421552No Hit
AAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT946590.3140835406010188No Hit
AAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG866450.2874926671037648Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA835330.277166887427766No Hit
AACGATGCCATCTCGCGATTCGGAGGGTAGATCGGAAGAGCACACGTCTG830150.2754481361834963Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG806830.2677104375316874Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT730960.24253637249254767No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC704270.2336804901161849No Hit
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACTGAGATCGGAAGAG676350.22441648727062302No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCAC639130.2120666955116039No Hit
AGCAGATATCACCTTATCCGGGATCCAGATCGGAAGAGCACACGTCTGAA636430.21117082131092277Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACGT599880.19904333907577637No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCT572690.19002155406965787Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACAGATCGGAAGAGCA572580.1899850554911116No Hit
TTTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTG539310.17894589450716303Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAAC516930.17152009279929498Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC489900.16255139663469834No Hit
ATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA468200.15535122250329814TruSeq Adapter, Index 10 (100% over 35bp)
GAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG452360.15009542719263552Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAACGATGCCATCTCGCGATTCGGAGGGTAGATCGGAAGAGCACACGTC440760.1462464861823018No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTC412180.13676349186546227No Hit
AGAAAAGTTACCACAGGGATAACTGGCTAGATCGGAAGAGCACACGTCTG394470.13088722071951306Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA383950.1273966293894518Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAACGATGCCATCTCGCGATTCGGAGGGTAGATCGGAAGAGCACACGTCT373760.12401553379502929Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAACAGATCGGAAGAGCA370970.12308979712099212No Hit
CATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC362210.12018318304767113No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACACG346750.11505347373562287No Hit
ACCGCCCTAGTTCTGACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT343630.1140182413259469Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGATCGTCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT325960.10815524239037819Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG325010.10784002739384223Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGTGTTCTTCGGAGCACAAGCGCGGTAACGCAAGATCGGAAGAGCACA320130.10622081772742595No Hit
TTTGCGTCAACTGTGGTCGTGACTTCTAGATCGGAAGAGCACACGTCTGA313400.10398776833091337Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGA308220.10226901708664364Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)