FastQCFastQC Report
Thu 14 Sept 2023
SRR14624514.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14624514.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27956394
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length64-75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA21035077.524242933477043No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA7960092.8473235854380934No Hit
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC7462122.6691997544461565No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA7357172.631659147456571Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA6426972.2989266784550253Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC5374081.9223080058179176No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCT5041311.803276202216924Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGA3168441.1333507461656178Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACTAGATCTCGTA2814691.0068143981659439TruSeq Adapter, Index 22 (97% over 38bp)
GATCGTCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCG2655420.9498435313223873TruSeq Adapter, Index 2 (100% over 35bp)
TTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC2450230.8764470839837212No Hit
ATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA2346990.8395181438636184Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACA1960780.7013708563414867No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC1880580.6726833224628326No Hit
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1875170.6707481658757564No Hit
TTTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGT1835690.6566261728891072No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC1574400.5631627598323303No Hit
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC1529920.5472522672273111Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC1524320.5452491476547369No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1422680.5088925274125125No Hit
TAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG1328730.47528661958334106Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCT792790.28358092248950273Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT732980.2621868900545614Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATGCCATCTCGCGATTCGGAGGGTAGATCGGAAGAGCACACGTCTGA727810.26033758144916685Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC638260.22830555328416102No Hit
AAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG617470.22086897187097876Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA590500.21122180492949127Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG571480.20441835238121198Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGA568300.2032808666239287Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCAGATATCACCTTATCCGGGATCCAGATCGGAAGAGCACACGTCTGAA556200.1989526975474734Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCT550310.1968458449970336Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC549350.1965024530703066No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACGT542980.19422390455650326No Hit
ATACCCCAACGCAAGGCGGGGTAGATCGGAAGAGCACACGTCTGAACTCC516170.18463396960280357Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACACG448580.16045703176167855No Hit
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC445080.15920508202881958No Hit
CATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC428250.15318499231338634No Hit
CCGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA425740.15228716550496463Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACAGATCGGAAGAGCA420470.1504020869072027No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCAC403010.14415664623985483No Hit
TAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG387740.138694568405353Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC381240.13636951890147206No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACACG380480.13609766695947983No Hit
AACGATGCCATCTCGCGATTCGGAGGGTAGATCGGAAGAGCACACGTCTG379810.13585800801061826Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTCTTTACTTATTCGGTTAAAAGAGAAGATCGGAAGAGCACACGTC362360.12961614434250712No Hit
AAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG358180.12812095866154982Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAAAAGTTACCACAGGGATAACTGGCTAGATCGGAAGAGCACACGTCTG355840.1272839408401527Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCAATGAGTTGAATACAAATGATTCTTCGAAGATCGGAAGAGCACACGTC347140.12417195150418898No Hit
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACTGAGATCGGAAGAG342860.12264099583086431No Hit
TCTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACA338550.12109930915982942No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC330700.11829136475898859No Hit
GTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC329320.11779773886431849No Hit
ACCTGCAGCTGGATCATCGAGATCGGAAGAGCACACGTCTGAACTCCAGT324570.11609866422686703Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC319850.1144103205871258No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAAC318390.11388807869856177Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATACCCCAACGCAAGGCGGGGTGCAGATCGGAAGAGCACACGTCTGAACT310680.1111302122870353Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG301660.10790375897549592Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAAAAGTTACCACAGGGATAACTGGAGATCGGAAGAGCACACGTCTGAA289570.10357916689827736Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTACCCCAACGCAAGGCGGGGTAGATCGGAAGAGCACACGTCTGAACTCC282500.10105022843790226Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)