FastQCFastQC Report
Thu 14 Sept 2023
SRR14624515.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14624515.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32186015
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length53-75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA17828205.539113804551449No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA8242742.560969414821934Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC7688872.388885359060449No Hit
ACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA7228802.2459443954152136Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA6867462.1336782450390332No Hit
AACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGA4304151.3372733468247002Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCT4250461.320592188874578Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC3740171.1620481752711542No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAGAAATCTCGTA3515971.0923905926222925TruSeq Adapter, Index 13 (97% over 38bp)
ATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA2005500.6230967083063871Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC1863440.5789595263657212No Hit
TTTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGT1754410.5450845654549034No Hit
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1694010.5263186511284482No Hit
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACA1544810.4799631144147543No Hit
TAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG1498810.46567119290785147Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1456030.45237970590643173No Hit
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC1418990.4408716021539169Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC1341090.41666854377592255No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACGT1340760.41656601477380784No Hit
AAAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCT1195540.37144703996440687Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC1183810.36780259998014664No Hit
GATCGTCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG1168350.36299927157804407TruSeq Adapter, Index 13 (100% over 35bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT1104810.3432577782617699Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC1081870.33613045914506656No Hit
TAAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCT884430.2747870464858728Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG881080.27374622176743535Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATACCCCAACGCAAGGCGGGGTGCAGATCGGAAGAGCACACGTCTGAACT846500.26300242512159394Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGATGCCATCTCGCGATTCGGAGGGTAGATCGGAAGAGCACACGTCTGA810700.25187958186187387Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG692030.21500953131352232Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCAGATATCACCTTATCCGGGATCCAGATCGGAAGAGCACACGTCTGAA658120.2044738996113685Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC631350.1961566226822426No Hit
AATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGA607670.1887993900456456Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT604990.1879667302708956No Hit
AAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC584030.1814545851668807No Hit
ATACCCCAACGCAAGGCGGGGTAGATCGGAAGAGCACACGTCTGAACTCC582480.18097300955088724Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA573050.17804316564197215Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT562980.1749144776077436No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC539990.16777162379375019No Hit
TCGACTCTTTACTTATTCGGTTAAAAGAGAAGATCGGAAGAGCACACGTC533310.16569618823579121No Hit
AACGATGCCATCTCGCGATTCGGAGGGTAGATCGGAAGAGCACACGTCTG526800.16367357064861868Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACAGATCGGAAGAGCA503590.1564623641665487No Hit
CTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC502070.15599010936892935No Hit
TTCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACG500200.15540911169027916No Hit
TTACCCCAACGCAAGGCGGGGTGCAGATCGGAAGAGCACACGTCTGAACT468140.1454482637878594Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCTGCAGCTGGATCATCGAGATCGGAAGAGCACACGTCTGAACTCCAGT454260.14113583182012437Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC454240.14112961794120832No Hit
CCGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA438970.13638532138880816Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACTGAGATCGGAAGAG422760.1313489725273539No Hit
AAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG395780.12296644986960951Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAACAGATCGGAAGAGCA391510.12163978672103397No Hit
TATACCCCAACGCAAGGCGGGGTGCAGATCGGAAGAGCACACGTCTGAAC389700.1210774306791319Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAAC385750.11985018959321309Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCAC384260.11938725561396774No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACACG372440.11571485317458531No Hit
AGAAAAGTTACCACAGGGATAACTGGCTAGATCGGAAGAGCACACGTCTG346220.10756845791565063Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAGAAATGTCGTA344030.10688803817434374TruSeq Adapter, Index 13 (97% over 38bp)
TAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG335930.10437141721334561Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCAATGAGTTGAATACAAATGATTCTTCGAAGATCGGAAGAGCACACGTC334940.1040638302070014No Hit
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC331220.10290804872861707No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)