FastQCFastQC Report
Thu 14 Sept 2023
SRR14624516.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14624516.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26253307
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length54-75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA17402776.628791565192149No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA8800443.352126267368907Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATCGTCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC8211593.1278307148124234TruSeq Adapter, Index 5 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTGAGCATCTCGTA6859202.6126994210672203TruSeq Adapter, Index 6 (97% over 36bp)
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC6469562.4642838328900813No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA6142752.339800467803923No Hit
ACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA5240641.996182804703423Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC4303941.6393896586056758No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCT4132201.5739731379364894Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC2319700.8835839233510658No Hit
AACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGA2150040.8189596838219277Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA2040520.7772430345632266Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACA1570530.5982217783077767No Hit
TTTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGT1560920.5945612870789955No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC1412160.5378979493897664No Hit
TAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG1390990.5298342033634086Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1363120.5192183978955489No Hit
TCTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC1181270.44995093380045414No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACGT1133880.4318998745567558No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC977180.37221215597714985No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA935750.3564312869232055Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCT934480.3559475383425029Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA903810.3442652005707319No Hit
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC775320.295322794953032Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCT706460.26909371836469975Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATGCCATCTCGCGATTCGGAGGGTAGATCGGAAGAGCACACGTCTGA682730.2600548570890516Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT678380.2583979229740467Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG662020.2521663270840508Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACG613820.23380673528100668No Hit
AGCAGATATCACCTTATCCGGGATCCAGATCGGAAGAGCACACGTCTGAA572770.21817060989687892Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGACTCTTTACTTATTCGGTTAAAAGAGAAGATCGGAAGAGCACACGTC534970.20377242379407667No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG488690.18614416842800033Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGATCGTCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT464730.17701769914167384Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG456750.1739780820755267Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC449060.17104892728371324No Hit
CTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC438790.1671370391547244No Hit
AAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT438450.16700753166067803No Hit
AATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGA434990.1656896024565591Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTGAGCATCGCGTA410660.15642219854435863TruSeq Adapter, Index 6 (97% over 36bp)
ATCGTCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCC408640.15565277166796548TruSeq Adapter, Index 6 (97% over 36bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAACAGATCGGAAGAGCA384080.1462977597450866No Hit
AAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC374450.14262965042842032No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCAC372560.1419097411232802No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACACG372510.1418906959035675No Hit
AGAAAAGTTACCACAGGGATAACTGGCTAGATCGGAAGAGCACACGTCTG365700.13929673697869757Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACTGAGATCGGAAGAG365570.13924721940744456No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT346120.13183862893920373No Hit
CCGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA340830.12982364469359994Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC333470.1270201883518903No Hit
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACAGATCGGAAGAGCA333010.12684497233053346No Hit
TTTGTGTTCTTCGGAGCACAAGCGCGGTAACGCAAGATCGGAAGAGCACA325010.12379773717650124No Hit
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC317280.1208533462089176No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAAGATCGGAAGAGCACACGTC312410.1189983418089005No Hit
ATACCCCAACGCAAGGCGGGGTAGATCGGAAGAGCACACGTCTGAACTCC310480.11826319632799022Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAAC308000.11731855343024024Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG307400.11709001079368783Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTG305300.11629011156575438Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG299460.11406562990331084Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC298070.11353617279529775No Hit
ACCTGCAGCTGGATCATCGAGATCGGAAGAGCACACGTCTGAACTCCAGT291720.11111742989178468Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC290880.1107974702006113No Hit
AGAAAAGTTACCACAGGGATAACTGGAGATCGGAAGAGCACACGTCTGAA289140.1101346965546093Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTTTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACAC287890.10965856606179175No Hit
GCTCGTCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTC282730.10769309938744098TruSeq Adapter, Index 5 (97% over 35bp)
AGAAAAGTTACCACAGGGATAACTGGCTTGAGATCGGAAGAGCACACGTC274050.10438684924531604No Hit
AACGATGCCATCTCGCGATTCGGAGGGTAGATCGGAAGAGCACACGTCTG269470.10264230711963258Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACA265830.10125581512454793No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)