FastQCFastQC Report
Thu 14 Sept 2023
SRR14624517.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14624517.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26857034
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length51-75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA14264065.311107697149283No Hit
ACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA8439183.142260608524381Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGA6728892.5054479210176375Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC6421012.3908112861606385No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA5398622.0101326155375165No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCT4414911.643856130948786Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA3647831.3582400796752165Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCT2972631.1068348053623493Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACTCCTATCTCGTA2787271.0378175043454165TruSeq Adapter, Index 4 (97% over 37bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC2215920.8250799399516715No Hit
TTTGTGTTCTTCGGAGCACAAGCGCGGTAACGCAAGATCGGAAGAGCACA2186410.8140921294585247No Hit
TAAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCT2106820.7844574348753477Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACGT1937260.721323136426755No Hit
TTTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGT1907920.7103986240625082No Hit
TTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC1857420.6915953563599018No Hit
TAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG1821160.6780942378074959Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA1715870.6388903555023984Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGACTCTTTACTTATTCGGTTAAAAGAGAAGATCGGAAGAGCACACGTC1499790.5584347102513255No Hit
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1284800.4783849177090813No Hit
AGAAAAGTTACCACAGGGATAACTGGCTAGATCGGAAGAGCACACGTCTG1200110.44685127925890844Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1196720.44558904010025824No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC1172500.43657091844170137No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA1054230.39253403782413204Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACA958250.3567966589311388No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT957550.35653601957684533Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG935620.34837056094876295Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATCGTCGGACTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG893670.3327508167878851TruSeq Adapter, Index 11 (100% over 35bp)
AGAAAAGTTACCACAGGGATAACTGGAGATCGGAAGAGCACACGTCTGAA874500.3256130218995888Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC837880.31197786025068885No Hit
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC835730.311177325091073Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAAAAGTTACCACAGGGATAACTGGCTTGAGATCGGAAGAGCACACGTC806780.300398026081361No Hit
TAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG795380.29615332802572314Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC752130.28004953934972865No Hit
TTTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTG736610.2742707925231058Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG677960.25243293805265316Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACG654970.24387279697378347No Hit
AAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT633370.23583021118415382No Hit
AATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGA631310.23506318679866137Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT590390.21982695483052966No Hit
ATACCCCAACGCAAGGCGGGGTAGATCGGAAGAGCACACGTCTGAACTCC580770.21624502541866686Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATACCCCAACGCAAGGCGGGGTGCAGATCGGAAGAGCACACGTCTGAACT544040.20256890615694942Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACTGAGATCGGAAGAG537930.2002938969359014No Hit
AGAAAAGTTACCACAGGGATAACTGGCAGATCGGAAGAGCACACGTCTGA537630.20018219435548987Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA496830.18499064341952282Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC495480.18448798180767095No Hit
ACGATGCCATCTCGCGATTCGGAGGGTAGATCGGAAGAGCACACGTCTGA494960.1842943640016243Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC488010.18170658755542404No Hit
AAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC481460.1792677478831058No Hit
ATTGTGTTCTTCGGAGCACAAGCGCGGTAACGCAAGATCGGAAGAGCACA471560.17558156272952552No Hit
GAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG470060.1750230498274679Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCAGATATCACCTTATCCGGGATCCAGATCGGAAGAGCACACGTCTGAA455840.16972834751596172Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAACAGATCGGAAGAGCA439020.16346555617422237No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC435430.16212884862863114No Hit
AGCTACCATTTGAGAGATTATAACTGAGATCGGAAGAGCACACGTCTGAA392000.1459580384043897Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCAATGAGTTGAATACAAATGATTCTTCGAAGATCGGAAGAGCACACGTC388920.14481122524549805No Hit
AACGATGCCATCTCGCGATTCGGAGGGTAGATCGGAAGAGCACACGTCTG385260.14344845376447748Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG378520.1409388691245653Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC368440.13718566242273814No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCAC359710.1339351173327628No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACACG347120.1292473323748259No Hit
TATACCCCAACGCAAGGCGGGGTAGATCGGAAGAGCACACGTCTGAACTC339530.12642125709041438Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG325880.1213387896816901Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGATGCCATCTCGCGATTCGGAGGAGATCGGAAGAGCACACGTCTGAAC321890.11985314536221683Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG317830.11834143710731423Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTC312270.11627121595035401No Hit
TTACCCCAACGCAAGGCGGGGTGCAGATCGGAAGAGCACACGTCTGAACT305740.11383982311672987Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG301180.11214194389447471Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTCTTTACTTATTCGGTTAAAAGAGAGATCGGAAGAGCACACGTCT299440.11149406892808789Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGATGCCATCTCGCGATTCGGAGGAGATCGGAAGAGCACACGTCTGAA297430.11074566163933068Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC296590.11043289441417842Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAAC295710.11010523351163796Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TATACCCCAACGCAAGGCGGGGTGCAGATCGGAAGAGCACACGTCTGAAC290130.10802756551598364Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTTGTGTTCTTCGGAGCACAAGCAGATCGGAAGAGCACACGTCTGAACTC289360.10774086222626073Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACAGATCGGAAGAGCA288310.10734990319482039No Hit
TTACCCCAACGCAAGGCGGGGTAGATCGGAAGAGCACACGTCTGAACTCC288260.10733128609808514Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCAGAAAAGTTACCACAGGGATAACTGGCTAGATCGGAAGAGCACACGTC287960.1072195835176736No Hit
TCAGAAAAGTTACCACAGGGATAACTGGAGATCGGAAGAGCACACGTCTG286600.1067131984864747Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCGCCCTAGTTCTGACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT271210.1009828561113636Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)