FastQCFastQC Report
Thu 14 Sept 2023
SRR14624518.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14624518.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23104848
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length62-75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA17083567.393928754692522No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA6586212.850574909646668No Hit
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC6454002.793353152550495No Hit
ACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA6320972.735776491583065Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGA4899122.1203861631117418Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCT4091861.7709962861473922Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC2604971.127456021351017No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGCATGATCTCGTA2530061.0950342542829106TruSeq Adapter, Index 7 (97% over 36bp)
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1767290.7649000763822381No Hit
TTTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGT1744200.7549065027391654No Hit
TTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC1725030.7466095427245398No Hit
TAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG1636360.7082323155729048Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCT1630590.7057350041861344Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1577370.6827008773223697No Hit
ATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA1493370.6463448709985021Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACA1127240.48788029248233966No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT1103270.47750584639206456Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC1102520.4771812391927443No Hit
TAAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCT1070660.46339192536562024Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA980140.42421400045566193Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGATGCCATCTCGCGATTCGGAGGGTAGATCGGAAGAGCACACGTCTGA962090.41640178719202137Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG921400.3987907646049002Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC904240.391363751884453No Hit
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACAGATCGGAAGAGCA816270.3532894914521835No Hit
AGAAAAGTTACCACAGGGATAACTGGCTAGATCGGAAGAGCACACGTCTG748590.3239969377855245Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG725970.31420678465402585Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATACCCCAACGCAAGGCGGGGTAGATCGGAAGAGCACACGTCTGAACTCC659330.28536435297042423Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AACGATGCCATCTCGCGATTCGGAGGGTAGATCGGAAGAGCACACGTCTG657610.28461992045998313Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC654280.28317866449500123No Hit
ATACCCCAACGCAAGGCGGGGTGCAGATCGGAAGAGCACACGTCTGAACT631090.2731418098920192Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC627690.27167025725510074No Hit
AAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC561190.24288841891537222No Hit
TAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG552310.23904506967542047Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC550580.23829630906898847No Hit
CTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC533980.23111166972403369No Hit
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACTGAGATCGGAAGAG533840.23105107638016056No Hit
AATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGA491240.21261338745877056Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC463620.20065918633180363No Hit
CCGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA403820.17477717230600262Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCAC389720.16867455695878197No Hit
AGAAAAGTTACCACAGGGATAACTGGAGATCGGAAGAGCACACGTCTGAA377950.1635803879774496Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTACCCCAACGCAAGGCGGGGTGCAGATCGGAAGAGCACACGTCTGAACT354200.15330115999897512Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG351290.15204168406561255Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTACCCCAACGCAAGGCGGGGTAGATCGGAAGAGCACACGTCTGAACTCC340540.14738898087535568Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACACG336080.14545865006339795No Hit
TATACCCCAACGCAAGGCGGGGTAGATCGGAAGAGCACACGTCTGAACTC329410.1425718100374432Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGAAAAGTTACCACAGGGATAACTGGCAGATCGGAAGAGCACACGTCTGA324670.14052029253773926Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG318630.13790612255921356Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCAATGAGTTGAATACAAATGATTCTTCGAAGATCGGAAGAGCACACGTC309130.13379443136782376No Hit
AGCAGATATCACCTTATCCGGGATCCAGATCGGAAGAGCACACGTCTGAA293480.12702096114200795Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATACCCCAACGCAAGGCGGGGTGCAGATCGGAAGAGCACACGTCTGAAC291860.1263198095914762Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG276360.11961126080552445Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC272660.1180098652888779Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACGT260320.1126689948360621No Hit
CAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG257590.11148742463053642Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTGTGTTCTTCGGAGCACAAGCGCGGTAACGCAAGATCGGAAGAGCACA251190.10871744319633699No Hit
TCGACTCTTTACTTATTCGGTTAAAAGAGAAGATCGGAAGAGCACACGTC249060.10779555875026747No Hit
AAAACGATGCCATCTCGCGATTCGGAGGGTAGATCGGAAGAGCACACGTC248640.10761377871864813No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC237960.10299137220032782No Hit
ACCGCCCTAGTTCTGACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT233140.10090522993269636Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG233060.10087060516476888Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT231340.10012617265432779No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)