FastQCFastQC Report
Thu 14 Sept 2023
SRR14624519.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14624519.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26958283
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length40-75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA11589424.299020082250787No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCTCTACATCTCGTA9346143.4668899350897084TruSeq Adapter, Index 1 (97% over 36bp)
ACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA7226622.6806677561772014Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC5431922.014935446741916No Hit
AACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGA5056931.8758353415905604Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA4670351.7324360012097209No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA3320331.2316548498285296Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCT3272451.2138940747821365Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCT1837800.6817199745250838Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG1764400.6544927212167037Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC1758930.6524636602412698No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC1687100.6258187882366247No Hit
TTTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGT1626760.6034360571109072No Hit
TAAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCT1570540.5825816132281124Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA1566320.5810162316346335Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTGTGTTCTTCGGAGCACAAGCGCGGTAACGCAAGATCGGAAGAGCACA1280980.4751712117570693No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1186010.43994270703367866No Hit
AAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG1174170.4355507359278037Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1085200.4025478922377957No Hit
AGAAAAGTTACCACAGGGATAACTGGCTAGATCGGAAGAGCACACGTCTG944630.3504043636606975Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC919410.3410491684503794Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT873490.3240154426748914Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC846570.3140296435051149No Hit
TCGACTCTTTACTTATTCGGTTAAAAGAGAAGATCGGAAGAGCACACGTC799330.29650627230228277No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACGT794330.2946515547744639No Hit
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACA751190.27864905194444317No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA744100.27601906248999614Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAAAAGTTACCACAGGGATAACTGGAGATCGGAAGAGCACACGTCTGAA739440.274290465754069Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTG687920.2551794563474239Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG658190.24415130592701323Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC602580.22352313758261236No Hit
ACGATGCCATCTCGCGATTCGGAGGGTAGATCGGAAGAGCACACGTCTGA534080.1981135074514946Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG528060.19588042754800075Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAAAAGTTACCACAGGGATAACTGGCTTGAGATCGGAAGAGCACACGTC506720.18796449313927002No Hit
AGCAGATATCACCTTATCCGGGATCCAGATCGGAAGAGCACACGTCTGAA488180.18108720054611788Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGTGTTCTTCGGAGCACAAGCGCGGTAACGCAAGATCGGAAGAGCACA468740.1738760587979583No Hit
CTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC459770.17054869555305135No Hit
CCGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA438340.16259937622881993Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGAAAAGTTACCACAGGGATAACTGGCAGATCGGAAGAGCACACGTCTGA422250.15663089522429896Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC401680.14900058731485236No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC399480.1481845116026121No Hit
GATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC395620.14675266967113595No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT389130.14434524632002713No Hit
AATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGA385420.14296904591438558Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AACGATGCCATCTCGCGATTCGGAGGGTAGATCGGAAGAGCACACGTCTG382210.14177831726152587Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT380500.14114400386701187No Hit
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACTGAGATCGGAAGAG379630.14082128301717137No Hit
GAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG358690.13305372601066617Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCAC329660.12228523604415015No Hit
ATACCCCAACGCAAGGCGGGGTAGATCGGAAGAGCACACGTCTGAACTCC327690.12155447733818953Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACG320420.11885771805274098No Hit
TCTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC311440.11552664537277837No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAAGATCGGAAGAGCACACGTC299990.11127934223407329No Hit
GAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG296450.10996620222437758Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC284640.10558535942366953No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCT282520.10479895919187435Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACACG277770.10303697754044648No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAACAGATCGGAAGAGCA272630.10113032792184874No Hit
CAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG271400.1006740674100053Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTACCATTTGAGAGATTATAACTGAGATCGGAAGAGCACACGTCTGAA269750.10006201062582509Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)