FastQCFastQC Report
Thu 14 Sept 2023
SRR14624520.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14624520.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25396744
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length64-75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA17524826.900419990846071No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA7407132.9165667850965464No Hit
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC6987992.751529881153269No Hit
ACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA6661352.6229149689424753Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCT6113282.407111714793046Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGA5246842.065949871369338Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA4067201.6014651326957503Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC3695361.4550526634437864No Hit
ATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA2641061.039920707945869Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC2341930.9221378929519469No Hit
TTTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGT2305890.9079470974704474No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA2204650.8680837197083217No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGAGAGGATCTCGTA2039790.8031698866594867TruSeq Adapter, Index 7 (97% over 37bp)
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1748320.6884032063322763No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACGT1603680.6314510238005313No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC1460090.5749122800938578No Hit
AAAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCT1443490.5683760091451093Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC1407080.5540395256966799No Hit
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACA1377320.5423214881403695No Hit
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC1340380.5277763165230944Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG1163820.45825559370917784Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCT1110840.43739465184985915Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATACCCCAACGCAAGGCGGGGTAGATCGGAAGAGCACACGTCTGAACTCC802010.315792449614801Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACAGATCGGAAGAGCA744700.2932265647911402No Hit
ACGATGCCATCTCGCGATTCGGAGGGTAGATCGGAAGAGCACACGTCTGA699600.27546838287616715Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGA688830.2712276817847201Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACTGAGATCGGAAGAG668560.26324634370453154No Hit
CTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC659880.2598285827506077No Hit
AGAAAAGTTACCACAGGGATAACTGGCTAGATCGGAAGAGCACACGTCTG658730.259375768799339Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG651700.25660769742767026Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTCTTTACTTATTCGGTTAAAAGAGAAGATCGGAAGAGCACACGTC625940.24646466491925106No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT621350.24465734662679592Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC606040.23862901480599247No Hit
TAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG560270.22060701954549766Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACGATGCCATCTCGCGATTCGGAGGGTAGATCGGAAGAGCACACGTCTG559430.2202762684854405Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCAGATATCACCTTATCCGGGATCCAGATCGGAAGAGCACACGTCTGAA532270.2095819842102594Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG489020.19255224212993602Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCAATGAGTTGAATACAAATGATTCTTCGAAGATCGGAAGAGCACACGTC468630.18452365389831074No Hit
TTTGTGTTCTTCGGAGCACAAGCGCGGTAACGCAAGATCGGAAGAGCACA454200.17884182318804331No Hit
TTCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACG439000.17285680400605682No Hit
CATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC438130.1725142404081405No Hit
AGAAAAGTTACCACAGGGATAACTGGAGATCGGAAGAGCACACGTCTGAA431430.1698761069529228Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTACCCCAACGCAAGGCGGGGTAGATCGGAAGAGCACACGTCTGAACTCC423270.16666309665522477Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TATACCCCAACGCAAGGCGGGGTAGATCGGAAGAGCACACGTCTGAACTC423060.1665804088902105Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC421490.1659622194089132No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACACG406930.16022920103458932No Hit
CCGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA400500.15769738042010426Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG379610.1494719165574926Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAAAAGTTACCACAGGGATAACTGGCTTGAGATCGGAAGAGCACACGTC374780.14757009796216397No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA366720.14439646279066323Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAACAGATCGGAAGAGCA353110.1390375081152135No Hit
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC347030.1366435004424189No Hit
TCTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC337310.13281623817604335No Hit
GAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG331960.1307096689244889Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATACCCCAACGCAAGGCGGGGTGCAGATCGGAAGAGCACACGTCTGAACT331390.1304852307051644Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC328860.12948904001237324No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT319870.12594921616723784No Hit
AAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC315680.1242993983795718No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCAC315510.12423246066503642No Hit
AAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT303470.11949169547088398No Hit
AGAAAAGTTACCACAGGGATAACTGGCAGATCGGAAGAGCACACGTCTGA283710.11171117053430156Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACACG275380.10843122252206817No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)