FastQCFastQC Report
Thu 14 Sept 2023
SRR14624521.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14624521.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30990433
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length48-75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA20137656.49802150231331No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA11389363.675121286624165Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA8193642.643925626983011No Hit
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC6861982.2142252739740678No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCT6733512.1727705450259442Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA5945761.9185791950696525Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC4379141.4130618955856473Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC4032081.3010724955020796No Hit
AACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGA3786171.2217222005255621Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA3201121.032938132874749Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC2506740.808875435848218No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA2409480.7774915568298126No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTACGCTATCTCGTA2293970.7402187636423151TruSeq Adapter, Index 6 (97% over 37bp)
TTTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGT2230870.7198576412275363No Hit
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1940960.6263094161995091No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC1840670.5939478160889201No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC1514480.4886927523729662No Hit
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACA1460640.47131964887357336No Hit
ATACCCCAACGCAAGGCGGGGTGCAGATCGGAAGAGCACACGTCTGAACT1306430.421559130845316Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATACCCCAACGCAAGGCGGGGTAGATCGGAAGAGCACACGTCTGAACTCC956770.30873076216779544Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACAGATCGGAAGAGCA919880.2968270885405183No Hit
AATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGA902120.29109628768336343Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCAGATATCACCTTATCCGGGATCCAGATCGGAAGAGCACACGTCTGAA788190.25433332925680646Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTACCCCAACGCAAGGCGGGGTGCAGATCGGAAGAGCACACGTCTGAACT788090.25430106123396207Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG765720.24708270452368317Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT762340.24599204535154448Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACGT689000.22232667739750522No Hit
GATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC674470.21763813367822257No Hit
CTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC672730.21707667008073103No Hit
ACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG624960.20166223556798965Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGATGCCATCTCGCGATTCGGAGGGTAGATCGGAAGAGCACACGTCTGA562660.1815592573359656Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TATACCCCAACGCAAGGCGGGGTGCAGATCGGAAGAGCACACGTCTGAAC539630.174127931674914Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACTGAGATCGGAAGAG539610.17412147807034514No Hit
AAAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCT503540.16248240223039156Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTTTCGAAGCATTAAGCGGAGCCAGATCGGAAGAGCACACGTCTGAAC495740.159965496448533Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG481770.15545765365717865Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC480320.1549897673259357No Hit
TTACCCCAACGCAAGGCGGGGTAGATCGGAAGAGCACACGTCTGAACTCC462600.14927187367791858Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACACG449070.14490601018707935No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC435570.1405498271030934No Hit
TAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG428580.138294292306274Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCAATGAGTTGAATACAAATGATTCTTCGAAGATCGGAAGAGCACACGTC423790.1367486540120301No Hit
TATACCCCAACGCAAGGCGGGGTAGATCGGAAGAGCACACGTCTGAACTC422470.1363227161104848Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACGATGCCATCTCGCGATTCGGAGGGTAGATCGGAAGAGCACACGTCTG417950.1348642014779206Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC416900.13452538723805504No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA413780.13351862492531164Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG370590.11958206585884101Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC365370.11789767506636645No Hit
TAAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCT364730.11769115972016268Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC358460.11566795468782252No Hit
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC356260.11495805818524704No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACACG351400.1133898322750121No Hit
CCGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA333110.10748801089678224Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCAC332540.1073040831665695No Hit
TCGACTCTTTACTTATTCGGTTAAAAGAGAAGATCGGAAGAGCACACGTC331630.107010444158686No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)