FastQCFastQC Report
Thu 14 Sept 2023
SRR14624522.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14624522.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29240395
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length63-75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA16971445.804107639448783No Hit
ACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA11094443.794216870189339Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGA8401062.8731007224765603Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCT7737932.646315140407645Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA6696822.2902631787292886No Hit
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC5564181.90290863033827No Hit
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA4331401.4813069385690583Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC3232081.1053475850787924No Hit
TTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC3129581.0702933390605702No Hit
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC2626510.8982470996031346Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA2615310.8944167819894362Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGGAATCTCGTA2487840.8508229796485307TruSeq Adapter, Index 3 (97% over 37bp)
TTTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGT2275080.7780606246940235No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1968060.6730620431085148No Hit
TAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG1678350.5739833541920347Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCT1632600.5583371907253647Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1618380.553474055326544No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC1590910.5440795173936603No Hit
AAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG1392500.4762247568817042Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCT1380050.4719669484629055Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACA1184990.40525786330861807No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC1178620.40307937016582707No Hit
ACGATGCCATCTCGCGATTCGGAGGGTAGATCGGAAGAGCACACGTCTGA1156650.39556579177538476Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGA895210.3061552349070524Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT848330.29012261975257175Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACGATGCCATCTCGCGATTCGGAGGGTAGATCGGAAGAGCACACGTCTG834060.285242384721547Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC804540.275146761868299No Hit
AGAAAAGTTACCACAGGGATAACTGGCTAGATCGGAAGAGCACACGTCTG792620.27107020955086275Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCAGATATCACCTTATCCGGGATCCAGATCGGAAGAGCACACGTCTGAA773490.2645278902696082Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG681190.23296196922100404Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAAAAGTTACCACAGGGATAACTGGAGATCGGAAGAGCACACGTCTGAA641540.21940196088322336Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA639810.21881031360896458Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG617920.21132409463004861Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC569540.19477849050944762No Hit
ATACCCCAACGCAAGGCGGGGTAGATCGGAAGAGCACACGTCTGAACTCC558080.19085925480828833Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAACT552590.18898171519228793Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTGTGTTCTTCGGAGCACAAGCGCGGTAACGCAAGATCGGAAGAGCACA552040.18879361923804383No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACGT517930.17712825014846756No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAAGATCGGAAGAGCACACGTC508530.1739135192941135No Hit
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACTGAGATCGGAAGAG467150.15976186368207407No Hit
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACAGATCGGAAGAGCA459940.1572960967182557No Hit
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA453590.1551244434283463Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC450620.15410872527542804No Hit
TGCAATTATTAGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTA434380.1485547647355653TruSeq Adapter, Index 3 (97% over 36bp)
AACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCT419300.1433975156628356Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC404230.13824368651654673No Hit
ATACCCCAACGCAAGGCGGGGTGCAGATCGGAAGAGCACACGTCTGAACT400140.13684493660225863Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTG390120.13341817030857483Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAAAAGTTACCACAGGGATAACTGGCAGATCGGAAGAGCACACGTCTGA384110.13136279451765273Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG376190.12865421277653738Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTCTTTACTTATTCGGTTAAAAGAGAAGATCGGAAGAGCACACGTC373100.1275974555063295No Hit
TTACCCCAACGCAAGGCGGGGTAGATCGGAAGAGCACACGTCTGAACTCC359240.12285743745937769Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACACG354640.12128427129660868No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC337470.11541225759775134No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC326230.11156826027828967No Hit
AAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC324680.11103817167996534No Hit
CGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAAC322020.11012847124671195Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC297650.10179411051047703No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)