FastQCFastQC Report
Thu 14 Sept 2023
SRR14624523.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14624523.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29600748
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length65-75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA16847535.691589280108732No Hit
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC9206123.1100970826818295No Hit
ACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA9114703.079212728002684Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGA9015193.045595334280066Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA5454221.842595329009929No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCT4165301.4071603866226623Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTCT3517071.1881692989650128Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCTG2831560.9565839349735351Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCTCAGGATCTCGTA2456270.829799976676265TruSeq Adapter, Index 15 (97% over 40bp)
AAAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCT2127480.7187250808661998Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCT1862260.6291259937079968Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG1832110.6189404402888737Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC1830500.6183965351145856No Hit
ACGATGCCATCTCGCGATTCGGAGGGTAGATCGGAAGAGCACACGTCTGA1792120.6054306465498778Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGT1766020.5966133017989951No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1650570.5576109090216234No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC1556660.5258853593834859No Hit
AACGATGCCATCTCGCGATTCGGAGGGTAGATCGGAAGAGCACACGTCTG1443100.48752146398462637Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACAGATCGGAAGAGCA1408910.47597108019027085No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTCT1403500.4741434236729424Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC1392920.4705691896704773No Hit
AAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG1382340.4669949556680122Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAA1353280.4571776361867612Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAACTGTGGTCGTGACTTCTAATTTGCTGGAGATCGGAAGAGCACACGTC1346100.45475202180701646No Hit
ATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGAA1321710.446512365160502Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACA1294730.4373977306249153No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCACAC1160090.39191239356518964No Hit
AGGTGACAGATGGTCCTTGCGGACGTTGACTGTCACTGAGATCGGAAGAG1075870.3634604098518051No Hit
TAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT937030.316556189728719No Hit
AAACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGT913110.3084753128535806No Hit
TCAATGAGTTGAATACAAATGATTCTTCGAGAGATCGGAAGAGCACACGT897260.303120718435899No Hit
TATACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACA894570.30221195761674674No Hit
AACTGTGGTCGTGACTTCTAATTTGCTGGTAGATCGGAAGAGCACACGTC839060.28345905312933306No Hit
TTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGAAC802600.2711417968221614Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG773440.26129069441083047Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAAGATCGGAAGAGCAC588330.19875511253972367No Hit
GATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC577900.1952315529323786No Hit
AGAAAAGTTACCACAGGGATAACTGGCTAGATCGGAAGAGCACACGTCTG549110.18550544736234367Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGAA529140.17875899622536565Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCAATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTC451690.1525941168783978No Hit
CATACCCCAACGCAAGGCGGGGTGCAATTATTAGAAGATCGGAAGAGCAC437780.1478949113042684No Hit
GAGACTATCCTTTGATTGAGTTTTTTGTAGATCGGAAGAGCACACGTCTG434570.14681047924870008Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGAGATCGGAAGAGCACAC422660.14278693227617087No Hit
AAAACGATGCCATCTCGCGATTCGGAGGGTAGATCGGAAGAGCACACGTC414200.139928896391402No Hit
AATGAGTTGAATACAAATGATTCTTCGAGATCGGAAGAGCACACGTCTGA409710.13841204283080955Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCAATGAGTTGAATACAAATGATTCTTCGAAGATCGGAAGAGCACACGTC401870.13576346111253676No Hit
TCGACTCTTTACTTATTCGGTTAAAAGAGAAGATCGGAAGAGCACACGTC401110.13550671084392868No Hit
AGCAGATATCACCTTATCCGGGATCCAGATCGGAAGAGCACACGTCTGAA375870.12697989929173412Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGAACAGATCGGAAGAGCA338110.11422346489352228No Hit
TATACCCCAACGCAAGGCGGGGTAGATCGGAAGAGCACACGTCTGAACTC338030.11419643854945827Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTTTTTTCGAAGCATTAAGCGGAGCCAAGATCGGAAGAGCACACGTCTGA335050.11318970723307396Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG306130.10341968385393505Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATACCCCAACGCAAGGCGGGGTAGATCGGAAGAGCACACGTCTGAACTCC299200.10107852679939035Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GAACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTG297240.10041638136982214Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAACGATGCCATCTCGCGATTCGGAGGGTAGATCGGAAGAGCACACGTCT296740.10024746671942208Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACGATGCCATCTCGCGATTCGGAGGGAGATCGGAAGAGCACACGTCTGA296520.10017314427324608Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)