FastQCFastQC Report
Thu 14 Sept 2023
SRR14714416_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14714416_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48375361
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length85
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAATCAA6302561.3028450578384314No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAATCA6300771.3024750347599474No Hit
AGCTAGGAATAATGAAAAAAAAAAAAAAATCAAGTAGATCGGAAGAGCGG2998960.6199354253914509No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAAATCAA2426290.5015549134610076No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAAAAA2396010.49529552864732107No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAAAAAAATCAAG2337410.4831819239550481No Hit
AGCTAGGAATAATGGAAAAAAAAAAAAAAATCAAGTAGATCGGAAGAGCG2239020.4628430576466396No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAAAAAAAAAAAAAAAT1920700.3970409647175553No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTGAAAAAAAAAAAAAAATCA1918610.39660892659798447No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGAAAAAAAAAAAAA1871640.38689943833184004No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAATCAA1795500.37116002090403005No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAAAAAATCAA1757790.3633647302394291No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAATCAA1751990.36216577277841916No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAAA1742990.3603053215458175No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAAAAAAAAAAAAAAATCAA1600470.33084404269355216No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAATCAAG1578890.32638309407138066No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAAAAATCAA1512900.31274185220033807No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAATCAAG1436160.29687840469035465No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAA1380220.28531466669571726No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAAA1338970.27678759854629303No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAATCAA1311060.27101813255719165No Hit
AGGAATAATGAAAAAAAAAAAAAAATCAAGTAGATCGGAAGAGCGGTTCA1293390.26736544663718376No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAAAT1284700.2655690776137051No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGTAAAAAAAAAAAAA1227490.2537428092784672No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAATCA1088670.2250463825996048No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1040710.2151322446978742No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGCAAAAAAAAAAAAA982540.20310752823115885No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCAAAAAAAAAAAAAAATCAAGT941800.194685885651582No Hit
AGGAATAATGGAAAAAAAAAAAAAAATCAAGTAGATCGGAAGAGCGGTTC935060.193292614395167No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAATCA840010.1736441822108573No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTAAAAAAAAAAAAAAATCA829400.17145091692442357No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAATCA827470.17105195349343233No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCAAAAAAAAAAAA812120.1678788505578284No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCAAAAAAAAAAAAAAATCA805600.1665310569982103No Hit
AGCTAGGAATAATGGAATAGGAAAAAAAAAAAAAAATCAAGTAGATCGGA744270.15385311543204813No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCGAAAAAAAAAAAAAAATCAA742630.15351409987410738No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAA642060.132724590933802No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAATCAAG629370.1301013546958337No Hit
AGAGCGCCGCCTGGATACCGCAGCTAGGAATAATGGAAAAAAAAAAAAAA626460.12949980879729248No Hit
AGAGCGCCGCCTGGATACCGCAGCTAGGAATAATGAAAAAAAAAAAAAAA610720.12624608630827583No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAATC578750.1196373500964675No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGAAAAAAAAAAAAAA569730.1177727645277934No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAAAAAAAAAAAAAAAA550350.11376659287359117No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAATAA550190.11373351818501158No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACAAAAAAAAAAAAAA528240.10919608434549975No Hit
CTCGCCCGTCACGTTGAACGCACGTTCAAAAAAAAAAAAAAATCAAGTAG517650.1070069533951385No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAAAAAAAAAAAAAAAT511780.10579352575787497No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAAAAAAAAAAAAAAAA509800.1053842264867026No Hit
AGCTAGGAATAATGAAAAAAAAAAAAAATCAAGTAGATCGGAAGAGCGGT502570.10388966399651262No Hit
AGCTAGGAATAATGGAAAAAAAAAAAAAATCAAGTAGATCGGAAGAGCGG494570.10223592956753336No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAAAAAAAAAAAAAAA490880.10147314456216668No Hit
AGAGGTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAA484480.10015015701898328No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)