FastQCFastQC Report
Thu 14 Sept 2023
SRR14714419_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR14714419_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42897719
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length85
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTGGGATTATGACTGAACGCCTCTAAGTCAGAATCCCGCCCAGGCG2446470.5703030503789724No Hit
GAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGAAAAAAAAAAAAA1909600.4451518739259773No Hit
GTGGGATTATGACTGAACGCCTCTAAGTCAGAATCCCGCCCAGGCGGAAC1761170.4105509665910208No Hit
CGTCTTGGGAAACGGGGTGCGGCCGGAAAGGGGGCCGCCCTCTCGCCCGT1692480.3945384601917878No Hit
CTGTGGGATTATGACTGAACGCCTCTAAGTCAGAATCCCGCCCAGGCGGA1530410.35675789661450297No Hit
AAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAAAA1474140.3436406490517596No Hit
AGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAAAAAAAAAAAAA1334990.3112030269021996No Hit
AATTATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCAAAAAAAA1322320.3082494899087758No Hit
GTCTTGGGAAACGGGGTGCGGCCGGAAAGGGGGCCGCCCTCTCGCCCGTC1320730.30787884083067446No Hit
TGTGGGATTATGACTGAACGCCTCTAAGTCAGAATCCCGCCCAGGCGGAA1301460.3033867604941885No Hit
CATCTGTGGGATTATGACTGAACGCCTCTAAGTCAGAATCCCGCCCAGGC1141650.2661330314555886No Hit
CAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAG1033170.2408449735987128No Hit
ACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAAAAAAAAAA1011330.23575379380894354No Hit
CGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA991410.23111019026442872No Hit
GAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGGCGGAGATGGGCG964890.22492804337685182No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCATAAGCTTGCGT951470.22179967191262545No Hit
GAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGTGC949030.22123087710094794No Hit
AATTATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCATAAGCTT912000.21259871649585843No Hit
AGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAAAGTC908630.21181312694038582No Hit
AAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAAAAAAAAAAAAAA892730.2081066361593725No Hit
GAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTAAAAAAAAAAAAAA885510.2064235629871136No Hit
TCGTCTTGGGAAACGGGGTGCGGCCGGAAAGGGGGCCGCCCTCTCGCCCG879970.20513211902945236No Hit
AGCGGGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAA830470.19359304395648635No Hit
GTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGT816790.19040406320904849No Hit
GATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAAAAAAAAAA778710.18152713434483544No Hit
TGGGATTATGACTGAACGCCTCTAAGTCAGAATCCCGCCCAGGCGGAACG765200.1783777827441128No Hit
GGCCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCAC701680.16357046863027846No Hit
AATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTG699060.16295971354560834No Hit
AAGTACCGTAAGGGAAAGTTGAAAAGAACTTTGAAGAGAGAGTTCAAGAG656140.15295451956314973No Hit
AAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATT646250.1506490356748339No Hit
GGGATTATGACTGAACGCCTCTAAGTCAGAATCCCGCCCAGGCGGAACGA645060.15037163164782724No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA602410.14042937807485753No Hit
CAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGATTTCTGC583770.1360841586938457No Hit
AGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAAAAAAAAAA583520.13602588053691153No Hit
GAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAAAAAAAA582270.13573448975224067No Hit
GGGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAGATGGTGAACT575520.13416097951501804No Hit
AAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAA549750.1281536670982436No Hit
ACGAGCGTACGCGTTAGGACCCGAAAGATGGTGAACTATGCCTGGGCAGG544630.12696013044423177No Hit
AGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGG517740.12069173188439226No Hit
TCTGTGGGATTATGACTGAACGCCTCTAAGTCAGAATCCCGCCCAGGCGG506540.11808087045374138No Hit
ACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAG476360.11104553134864815No Hit
AGAGCGTCTTGGGAAACGGGGTGCGGCCGGAAAGGGGGCCGCCCTCTCGC462910.10791016650558974No Hit
GGGAGGTGGAGCACGAGCGTACGCGTTAGGACCCGAAAGATGGTGAACTA448550.1045626691712909No Hit
AATTATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCATAAAAAA430060.10025241668443956No Hit
AGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTT430040.10024775443188483No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)